PMID- 19767822
OWN - NLM
STAT- MEDLINE
DCOM- 20091127
LR  - 20161125
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 87
IP  - 4
DP  - 2009 Aug
TI  - Involvement of a disintegrin and metalloproteinase 10 and 17 in shedding of tumor 
      necrosis factor-alpha.
PG  - 581-93
LID - 10.1139/o09-015 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is initially synthesized as a 
      membrane-bound protein and converted into a soluble form by proteolytic cleavage. 
      Although a disintegrin and metalloproteinase 17 (ADAM17) is considered to be the 
      primary sheddase for TNF-alpha, it is not known whether ADAM17 is solely 
      responsible for that process in any type of cells. To identify the TNF-alpha 
      sheddase(s) in varieties of cells, we performed experiments using a unique 
      screening system and observed that ADAM9, ADAM10, ADAM17, and ADAM19 were capable 
      of cleaving TNF-alpha. We then performed RNA interference experiments and 
      confirmed that ADAM10 and ADAM17 were in fact involved in TNF-alpha shedding in 
      293A cells. In mouse macrophages, ADAM17 was confirmed to be the primary 
      sheddase, but the involvement of ADAM10 was also demonstrated. In NIH3T3 cells, 
      ADAM10 could be more important in the shedding than ADAM17. In mouse vascular 
      endothelial cell line UVfemale2, ADAM10 and ADAM17 were equally involved in 
      TNF-alpha shedding, whereas ADAM17 was a major sheddase in human osteoarthritic 
      chondrocytes. From these observations and others, we concluded that both ADAM10 
      and ADAM17 can be a TNF-alpha sheddase and that their significance could be 
      determined by their expression levels and the abundance of tissue inhibitor of 
      metalloproteinases.
FAU - Hikita, Atsuhiko
AU  - Hikita A
AD  - Department of Pathomechanisms, Clinical Research Center, National Hospital 
      Organization Sagamihara Hospital, Sagamihara, Kanagawa 228-8522, Japan.
FAU - Tanaka, Nobuho
AU  - Tanaka N
FAU - Yamane, Shoji
AU  - Yamane S
FAU - Ikeda, Yasuko
AU  - Ikeda Y
FAU - Furukawa, Hiroshi
AU  - Furukawa H
FAU - Tohma, Shigeto
AU  - Tohma S
FAU - Suzuki, Ryuji
AU  - Suzuki R
FAU - Tanaka, Sakae
AU  - Tanaka S
FAU - Mitomi, Hiroyuki
AU  - Mitomi H
FAU - Fukui, Naoshi
AU  - Fukui N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (DNA Primers)
RN  - 0 (Membrane Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.- (Amyloid Precursor Protein Secretases)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.81 (ADAM10 Protein)
RN  - EC 3.4.24.81 (Adam10 protein, mouse)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
RN  - EC 3.4.24.86 (Adam17 protein, mouse)
SB  - IM
MH  - ADAM Proteins/*physiology
MH  - ADAM10 Protein
MH  - ADAM17 Protein
MH  - Amyloid Precursor Protein Secretases/*physiology
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA Primers
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Macrophages/metabolism
MH  - Membrane Proteins/*physiology
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2009/09/22 06:00
MHDA- 2009/12/16 06:00
CRDT- 2009/09/22 06:00
PHST- 2009/09/22 06:00 [entrez]
PHST- 2009/09/22 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - o09-015 [pii]
AID - 10.1139/o09-015 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2009 Aug;87(4):581-93. doi: 10.1139/o09-015.