PMID- 19770597
OWN - NLM
STAT- MEDLINE
DCOM- 20100118
LR  - 20090922
IS  - 1528-1159 (Electronic)
IS  - 0362-2436 (Linking)
VI  - 34
IP  - 16
DP  - 2009 Jul 15
TI  - Matrix metalloproteinase-3 on ligamentum flavum in degenerative lumbar 
      spondylolisthesis.
PG  - E552-7
LID - 10.1097/BRS.0b013e3181aa0232 [doi]
AB  - STUDY DESIGN: Human ligamentum flavum (LF) was examined for the activity level of 
      matrix metalloproteinase-3 (MMP-3) in degenerative spondylolithesis (DS) patients 
      using immunohistochemistry, Western blot, reverse transcriptase-polymerase chain 
      reaction (RT-PCR), and quantitative real-time PCR. OBJECTIVE: To investigate the 
      hypothesis that the activity of MMP-3 is elevated in LF of DS patients, which 
      might contribute to DS pathogenesis. SUMMARY OF BACKGROUND DATA: MMP-3 is a 
      proteinase produced by connective tissue cells and is responsible for the 
      degradation and modification of extracellular matrix molecules. MMP-3 activity 
      has been established in articular cartilage, synovial membrane, and 
      intervertebral discs, but not in the LF. METHODS: The experimental group 
      consisted of 18 patients with DS and the control group consisted of 18 patients 
      with spinal stenosis (SS) without any instabilities. MMP-3 expression was 
      measured with in situ using immunohistochemistry and both for mRNA and protein 
      levels. RESULTS: The MMP-3 positive cell ratio in the LF observed in DS patients 
      was substantially higher than in SS patients (P = 0.030). In Western blot, the 
      average optical density (OD) of MMP-3 was higher in LF of DS than of SS (P = 
      0.028). There was greater MMP-3 expression in DS patients as quantified by RT-PCR 
      (P = 0.004). CONCLUSION: Our study shows that MMP-3 expression in the LF of DS 
      patients was significantly higher than in SS patients. Increased MMP-3 expression 
      may be associated with the degenerative changes of LF in DS patients comprising 
      one of the mechanisms of pathogenesis in DS.
FAU - Oh, In-Soo
AU  - Oh IS
AD  - Department of Orthopaedic Surgery, Kang-Nam St Mary's Hospital, College of 
      Medicine, The Catholic University of Korea, Seoul, Korea.
FAU - Ha, Kee-Yong
AU  - Ha KY
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Spine (Phila Pa 1976)
JT  - Spine
JID - 7610646
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Aged
MH  - Blotting, Western
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Ligamentum Flavum/*enzymology/metabolism
MH  - Lumbar Vertebrae/enzymology/metabolism/pathology
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spinal Stenosis/*enzymology/genetics
MH  - Spondylolisthesis/*enzymology/genetics
EDAT- 2009/09/23 06:00
MHDA- 2010/01/19 06:00
CRDT- 2009/09/23 06:00
PHST- 2009/09/23 06:00 [entrez]
PHST- 2009/09/23 06:00 [pubmed]
PHST- 2010/01/19 06:00 [medline]
AID - 00007632-200907150-00022 [pii]
AID - 10.1097/BRS.0b013e3181aa0232 [doi]
PST - ppublish
SO  - Spine (Phila Pa 1976). 2009 Jul 15;34(16):E552-7. doi: 
      10.1097/BRS.0b013e3181aa0232.