PMID- 19771174
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130704
LR  - 20211020
IS  - 1874-1924 (Electronic)
IS  - 1874-1924 (Linking)
VI  - 3
DP  - 2009 Aug 31
TI  - Gender-specific modulation of the response to arterial injury by soluble 
      guanylate cyclase alpha1.
PG  - 98-104
LID - 10.2174/1874192400903010098 [doi]
AB  - OBJECTIVE: Soluble guanylate cyclase (sGC), a heterodimer composed of alpha and 
      beta subunits, synthesizes cGMP in response to nitric oxide (NO). NO modulates 
      vascular tone and structure but the relative contributions of cGMP-dependent 
      versus cGMP-independent mechanisms remain uncertain. We studied the response to 
      vascular injury in male (M) and female (F) mice with targeted deletion of exon 6 
      of the sGCalpha1 subunit (sGCalpha1(-/-)), resulting in a non-functional heterodimer. 
      METHODS: We measured aortic cGMP levels and mRNA transcripts encoding sGC alpha1, alpha2, 
      and beta1 subunits in wild type (WT) and sGCa1(-/-) mice. To study the response to 
      vascular injury, BrdU-incorporation and neointima formation (maximum intima to 
      media (I/M) ratio) were determined 5 and 28 days after carotid artery ligation, 
      respectively. RESULTS: Aortic cGMP levels were 4-fold higher in F than in M mice 
      in both genotypes, and, within each gender, 4-fold higher in WT than in 
      sGCa1(-/-). In contrast, sGCalpha1, sGCalpha2, and sGCbeta1 mRNA expression did not differ 
      between groups. (3)H-thymidine incorporation in cultured sGCa1(-/-) smooth muscle 
      cells (SMC) was 27%+/-12% lower than in WT SMC and BrdU-incorporation in carotid 
      arteries 5 days after ligation was significantly less in sGCa1(-/-) M than in WT 
      M. Neointima area and I/M 28 days after ligation were 65% and 62% lower in 
      sGCa1(-/-) M than in WT M mice (p<0,05 for both) but were not different in F 
      mice. CONCLUSION: Functional deletion of sGCa1 resulted in reduced cGMP levels in 
      male sGCa1(-/-) mice and a gender-specific effect on the adaptive response to 
      vascular injury.
FAU - Vermeersch, Pieter
AU  - Vermeersch P
AD  - Vesalius Research Center (VIB) and Cardiac Unit.
FAU - Buys, Emmanuel
AU  - Buys E
FAU - Sips, Patrick
AU  - Sips P
FAU - Pokreisz, Peter
AU  - Pokreisz P
FAU - Marsboom, Glenn
AU  - Marsboom G
FAU - Gillijns, Hilde
AU  - Gillijns H
FAU - Pellens, Marijke
AU  - Pellens M
FAU - Dewerchin, Mieke
AU  - Dewerchin M
FAU - Bloch, Kenneth D
AU  - Bloch KD
FAU - Brouckaert, Peter
AU  - Brouckaert P
FAU - Janssens, Stefan
AU  - Janssens S
LA  - eng
GR  - R01 HL070896/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20090831
PL  - United Arab Emirates
TA  - Open Cardiovasc Med J
JT  - The open cardiovascular medicine journal
JID - 101480504
PMC - PMC2743853
OTO - NOTNLM
OT  - Soluble guanylate cyclase
OT  - gender
OT  - nitric oxide.
OT  - vascular remodelling
EDAT- 2009/09/23 06:00
MHDA- 2009/09/23 06:01
PMCR- 2009/01/01
CRDT- 2009/09/23 06:00
PHST- 2009/07/13 00:00 [received]
PHST- 2009/07/24 00:00 [revised]
PHST- 2009/07/27 00:00 [accepted]
PHST- 2009/09/23 06:00 [entrez]
PHST- 2009/09/23 06:00 [pubmed]
PHST- 2009/09/23 06:01 [medline]
PHST- 2009/01/01 00:00 [pmc-release]
AID - TOCMJ-3-98 [pii]
AID - 10.2174/1874192400903010098 [doi]
PST - epublish
SO  - Open Cardiovasc Med J. 2009 Aug 31;3:98-104. doi: 10.2174/1874192400903010098.