PMID- 19772927
OWN - NLM
STAT- MEDLINE
DCOM- 20100212
LR  - 20201209
IS  - 1095-8274 (Electronic)
IS  - 1075-9964 (Linking)
VI  - 15
IP  - 6
DP  - 2009 Dec
TI  - Comparison of VIDAS CDAB and CDA immunoassay for the detection of Clostridium 
      difficile in a tcdA- tcdB+ C. difficile prevalent area.
PG  - 266-9
LID - 10.1016/j.anaerobe.2009.09.008 [doi]
AB  - Enzyme immunoassays for TcdA and/or TcdB are widely used for diagnosis of C. 
      difficile infection. This study compared the performance of the new VIDAS C. 
      difficile Toxin A & B assay (CDAB) with that of the existing VIDAS C. difficile 
      Toxin A II assay (CDA) in a tcdA(-)tcdB(+) prevalent area. A total of 555 fecal 
      samples were cultured and tested using CDAB and CDA. C. difficile was isolated in 
      150 samples and the concordance rate was 81.8% (454/555) between CDAB and CDA. 
      PCR assays for tcdA and/or tcdB were used as a confirmatory test on C. difficile 
      strains recovered from culture positive cases (n=150) and on fecal specimens in 
      culture negative/CDAB positive or equivocal cases (n=27). The number of 
      tcdA(+)tcdB(+), tcdA(-)tcdB(+), and tcdA(-)tcdB(-) strains on culture positive 
      isolates (n=150) were 75 (50.0%), 41 (27.3%), and 34 (22.7%), respectively. PCR 
      assays for tcdB gene alone in stool specimens (n=27) showed positivity in five 
      cases. The sensitivity of VIDAS CDAB was higher than that of VIDAS CDA (65.3% vs. 
      29.8%), by more than 2-fold. The specificity of CDAB was almost the same as CDA 
      (93.8% vs. 94.5%). Toxigenic culture of C. difficile isolates in culture 
      positive/VIDAS CDAB negative cases (n=62) additionally detected 22 VIDAS CDAB 
      positive and 9 VIDAS CDAB equivocal cases. The VIDAS CDAB assay detects more 
      tcdA(+)tcdB(+) strains (60% vs. 45.3%) and tcdA(-)tcdB(+) strains (70.7% vs. 0%) 
      compared with VIDAS CDA.
FAU - Shin, Bo-Moon
AU  - Shin BM
AD  - Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University, Seoul, 
      139 707, Korea. bmshin@unitel.co.kr
FAU - Lee, Eun-Joo
AU  - Lee EJ
FAU - Kuak, Eun-Young
AU  - Kuak EY
FAU - Yoo, Soo Jin
AU  - Yoo SJ
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20090920
PL  - England
TA  - Anaerobe
JT  - Anaerobe
JID - 9505216
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 0 (tcdA protein, Clostridium difficile)
RN  - 0 (toxB protein, Clostridium difficile)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bacterial Proteins/*analysis/genetics
MH  - Bacterial Toxins/*analysis/genetics
MH  - Child
MH  - *Clostridioides difficile/genetics/isolation & purification/metabolism
MH  - Culture Media
MH  - DNA, Bacterial/analysis
MH  - Enterocolitis, Pseudomembranous/*diagnosis/epidemiology/microbiology
MH  - Enterotoxins/*analysis/genetics
MH  - Feces/chemistry/microbiology
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques/*methods
MH  - Korea/epidemiology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Reagent Kits, Diagnostic
MH  - Sensitivity and Specificity
MH  - Young Adult
EDAT- 2009/09/24 06:00
MHDA- 2010/02/13 06:00
CRDT- 2009/09/24 06:00
PHST- 2009/04/14 00:00 [received]
PHST- 2009/08/28 00:00 [revised]
PHST- 2009/09/11 00:00 [accepted]
PHST- 2009/09/24 06:00 [entrez]
PHST- 2009/09/24 06:00 [pubmed]
PHST- 2010/02/13 06:00 [medline]
AID - S1075-9964(09)00131-0 [pii]
AID - 10.1016/j.anaerobe.2009.09.008 [doi]
PST - ppublish
SO  - Anaerobe. 2009 Dec;15(6):266-9. doi: 10.1016/j.anaerobe.2009.09.008. Epub 2009 
      Sep 20.