PMID- 19774472
OWN - NLM
STAT- MEDLINE
DCOM- 20100308
LR  - 20211020
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 17
IP  - 7
DP  - 2009
TI  - Assignment of genetic linkage maps to diploid Solanum tuberosum pachytene 
      chromosomes by BAC-FISH technology.
PG  - 899-915
LID - 10.1007/s10577-009-9077-3 [doi]
AB  - A cytogenetic map has been developed for diploid potato (Solanum tuberosum), in 
      which the arms of the 12 potato bivalents can be identified in pachytene 
      complements using multicolor fluorescence in situ hybridization (FISH) with a set 
      of 60 genetically anchored bacterial artificial chromosome (BAC) clones from the 
      RHPOTKEY BAC library. This diagnostic set of selected BACs (five per chromosome) 
      hybridizes to euchromatic regions and corresponds to well-defined loci in the 
      ultradense genetic map, and with these probes a new detailed and reliable 
      pachytene karyotype could be established. Chromosome size has been estimated both 
      from microscopic length measurements and from 4',6-diamidino-2-phenylindole 
      fluorescence-based DNA content measurements. In both approaches, chromosome 1 is 
      the largest (100-115 Mb) and chromosome 11 the smallest (49-53 Mb). Detailed 
      measurements of mega-base-pair to micrometer ratios have been obtained for 
      chromosome 5, with average values of 1.07 Mb/mum for euchromatin and 3.67 Mb/mum 
      for heterochromatin. In addition, our FISH results helped to solve two 
      discrepancies in the potato genetic map related to chromosomes 8 and 12. Finally, 
      we discuss the significance of the potato cytogenetic map for extended FISH 
      studies in potato and related Solanaceae, which will be especially beneficial for 
      the potato genome-sequencing project.
FAU - Tang, Xiaomin
AU  - Tang X
AD  - Wageningen UR Plant Breeding, Wageningen University and Research Center, 6708 PB, 
      Wageningen, The Netherlands.
FAU - de Boer, Jan M
AU  - de Boer JM
FAU - van Eck, Herman J
AU  - van Eck HJ
FAU - Bachem, Christian
AU  - Bachem C
FAU - Visser, Richard G F
AU  - Visser RG
FAU - de Jong, Hans
AU  - de Jong H
LA  - eng
PT  - Journal Article
DEP - 20090923
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
SB  - IM
MH  - Chromosomes, Artificial, Bacterial
MH  - *Chromosomes, Plant
MH  - Diploidy
MH  - *Genetic Linkage
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Solanum tuberosum/*genetics
PMC - PMC2776164
EDAT- 2009/09/24 06:00
MHDA- 2010/03/10 06:00
PMCR- 2009/09/23
CRDT- 2009/09/24 06:00
PHST- 2009/04/22 00:00 [received]
PHST- 2009/08/20 00:00 [accepted]
PHST- 2009/09/24 06:00 [entrez]
PHST- 2009/09/24 06:00 [pubmed]
PHST- 2010/03/10 06:00 [medline]
PHST- 2009/09/23 00:00 [pmc-release]
AID - 9077 [pii]
AID - 10.1007/s10577-009-9077-3 [doi]
PST - ppublish
SO  - Chromosome Res. 2009;17(7):899-915. doi: 10.1007/s10577-009-9077-3. Epub 2009 Sep 
      23.