PMID- 19775202
OWN - NLM
STAT- MEDLINE
DCOM- 20101027
LR  - 20211122
IS  - 1523-4681 (Electronic)
IS  - 0884-0431 (Linking)
VI  - 25
IP  - 3
DP  - 2010 Mar
TI  - Genome-wide expression analyses establish dendritic cells as a new osteoclast 
      precursor able to generate bone-resorbing cells more efficiently than monocytes.
PG  - 661-72
LID - 10.1359/jbmr.090829 [doi]
AB  - Dendritic cells (DCs), mononuclear cells that initiate immune responses, and 
      osteoclasts (OCs), multinucleated bone-resorbing cells, are hematopoietic cells 
      derived from monocytic precursor cells. Using in vitro generated dendritic cells, 
      we previously showed that human and murine DCs could transdifferentiate into 
      resorbing osteoclasts in the presence of macrophage colony-stimulating factor 
      (M-CSF) and receptor activator of NF-kappaB ligand (RANKL). In this study we 
      globally compared by transcriptomic profiling this new osteoclast differentiation 
      pathway from DCs with the canonical differentiation pathway from monocytes. DNA 
      chip data revealed that starting from two very distinct cell types, treatment 
      with M-CSF and RANKL generated two highly similar types of osteoclast. In 
      particular, DC-derived osteoclasts expressed all the characteristic marker genes 
      of monocyte-derived osteoclasts. Two major molecular events could be observed 
      during osteoclastogenesis: downregulation of a large set of monocyte or DC 
      specific markers, together with upregulation of characteristic osteoclast marker 
      genes. Most interestingly, our transcriptomic data showed a closer molecular 
      profile between DCs and OCs than between monocytes and OCs. Our data establish 
      DCs as a new osteoclast precursor able to generate OCs more efficiently than 
      monocytes.
CI  - Copyright 2010 American Society for Bone and Mineral Research.
FAU - Gallois, A
AU  - Gallois A
AD  - Institut de Genomique Fonctionnelle de Lyon, Universite de Lyon, Universite Lyon 
      1, CNES, INRA, Ecole Normale Superieure de Lyon, France.
FAU - Lachuer, J
AU  - Lachuer J
FAU - Yvert, G
AU  - Yvert G
FAU - Wierinckx, A
AU  - Wierinckx A
FAU - Brunet, F
AU  - Brunet F
FAU - Rabourdin-Combe, C
AU  - Rabourdin-Combe C
FAU - Delprat, C
AU  - Delprat C
FAU - Jurdic, P
AU  - Jurdic P
FAU - Mazzorana, M
AU  - Mazzorana M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (Antigens, Surface)
SB  - IM
MH  - Antigens, Surface/genetics/metabolism
MH  - *Bone Resorption
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - Genome-Wide Association Study
MH  - Humans
MH  - Monocytes/*cytology
MH  - Osteoclasts/*cytology
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2009/09/25 06:00
MHDA- 2010/10/28 06:00
CRDT- 2009/09/25 06:00
PHST- 2009/09/25 06:00 [entrez]
PHST- 2009/09/25 06:00 [pubmed]
PHST- 2010/10/28 06:00 [medline]
AID - 10.1359/jbmr.090829 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2010 Mar;25(3):661-72. doi: 10.1359/jbmr.090829.