PMID- 19775313
OWN - NLM
STAT- MEDLINE
DCOM- 20091230
LR  - 20230124
IS  - 1600-6143 (Electronic)
IS  - 1600-6135 (Linking)
VI  - 9
IP  - 11
DP  - 2009 Nov
TI  - Memory T-cell predominance following T-cell depletional therapy derives from 
      homeostatic expansion of naive T cells.
PG  - 2615-23
LID - 10.1111/j.1600-6143.2009.02820.x [doi]
AB  - T-cell depletion reportedly leads to alterations in the T-cell compartment with 
      predominant survival of memory phenotype CD4 T cells. Here, we asked whether the 
      prevalence of memory T cells postdepletion results from their inherent resistance 
      to depletion and/or to the homeostatic expansion of naive T cells and their 
      phenotypic conversion to memory, which is known to occur in lymphopenic 
      conditions. Using a 'mosaic memory' mouse model with trackable populations of 
      alloreactive memory T cells, we found that treatment with murine antithymocyte 
      globulin (mATG) or antilymphocyte serum (ALS) effectively depleted alloreactive 
      memory CD4 T cells, followed by rapid homeostatic proliferation of endogenous CD4 
      T cells peaking at 4 days postdepletion, with no homeostatic advantage to the 
      antigen-specific memory population. Interestingly, naive (CD44lo) CD4 T cells 
      exhibited the greatest increase in homeostatic proliferation following mATG 
      treatment, divided more extensively compared to memory (CD44hi) CD4 T cells and 
      converted to a memory phenotype. Our results provide novel evidence that memory 
      CD4 T cells are susceptible to lymphodepletion and that the postdepletional 
      T-cell compartment is repopulated to a significant extent by homeostatically 
      expanded naive T cells in a mouse model, with important important implications 
      for immune alterations triggered by induction therapy.
FAU - Sener, A
AU  - Sener A
AD  - Division of Transplantation, Department of Surgery, University of Maryland School 
      of Medicine, Baltimore, MD, USA.
FAU - Tang, A L
AU  - Tang AL
FAU - Farber, D L
AU  - Farber DL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090922
PL  - United States
TA  - Am J Transplant
JT  - American journal of transplantation : official journal of the American Society of 
      Transplantation and the American Society of Transplant Surgeons
JID - 100968638
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antilymphocyte Serum)
RN  - 0 (Isoantigens)
RN  - D7RD81HE4W (thymoglobulin)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology/pharmacology
MH  - Antilymphocyte Serum
MH  - CD4 Lymphocyte Count
MH  - CD4-Positive T-Lymphocytes/*cytology/*immunology
MH  - Cell Division/immunology
MH  - Homeostasis/immunology
MH  - Immunologic Memory/*immunology
MH  - Immunophenotyping
MH  - Isoantigens/immunology
MH  - *Leukocyte Reduction Procedures
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - *Transplantation Immunology
EDAT- 2009/09/25 06:00
MHDA- 2009/12/31 06:00
CRDT- 2009/09/25 06:00
PHST- 2009/09/25 06:00 [entrez]
PHST- 2009/09/25 06:00 [pubmed]
PHST- 2009/12/31 06:00 [medline]
AID - S1600-6135(22)01889-5 [pii]
AID - 10.1111/j.1600-6143.2009.02820.x [doi]
PST - ppublish
SO  - Am J Transplant. 2009 Nov;9(11):2615-23. doi: 10.1111/j.1600-6143.2009.02820.x. 
      Epub 2009 Sep 22.