PMID- 19776366
OWN - NLM
STAT- MEDLINE
DCOM- 20100222
LR  - 20131121
IS  - 1522-1598 (Electronic)
IS  - 0022-3077 (Linking)
VI  - 102
IP  - 6
DP  - 2009 Dec
TI  - Intraperiaqueductal gray glycine and D-serine exert dual effects on rostral 
      ventromedial medulla ON- and OFF-cell activity and thermoceptive threshold in the 
      rat.
PG  - 3169-79
LID - 10.1152/jn.00124.2009 [doi]
AB  - We have studied the involvement of the N-methyl-D-aspartate receptor (NMDAR) 
      glycine site and the strychnine-sensitive glycine receptor (GlyR) in the 
      ventrolateral periaqueductal gray (VL-PAG) on nociceptive behavior (tail flick) 
      and pain-related changes on neuronal activity in the rostral ventromedial medulla 
      (RVM). Glycine or D-serine increased the tail-flick latency, reduced OFF-cell 
      pause, and delayed its onset and increased the time between the onset of the 
      OFF-cell pause and the tail withdrawal. Conversely, they decreased the ongoing 
      activity of the ON cell, the tail-flick-induced ON-cell firing, whereas they 
      delayed the onset of increased tail-flick-induced ON-cell firing. Also, glycine 
      or D-serine reduced the interval between the onset of the increased ON-cell 
      firing and tail withdrawal. Whereas 7-Cl-kynurenic acid (7-Cl-KYN) prevented such 
      effects, strychnine did not do so. A higher dose of 7-Cl-KYN or strychnine was 
      per se able to reduce or increase tail-flick latency and increase or reduce 
      ON-cell activities, respectively. A higher dose of glycine was hyperalgesic in 
      the presence of 7-Cl-KYN, whereas such an effect was prevented by strychnine. 
      These data suggest 1) a dual role of glycine in producing hyperalgesia or 
      analgesia by stimulating the GlyR or the NMDARs within the VL-PAG, respectively; 
      2) consistently that RVM ON and OFF cells display opposite firing patterns to the 
      stimulation of the VL-PAG NMDAR glycine site and GlyR activation; and 3) a tonic 
      role of these receptors within the VL-PAG-RVM antinociceptive descending pathway.
FAU - Palazzo, Enza
AU  - Palazzo E
AD  - Department of Experimental Medicine, Second University of Naples, Naples, Italy.
FAU - Guida, Francesca
AU  - Guida F
FAU - Migliozzi, Annalucia
AU  - Migliozzi A
FAU - Gatta, Luisa
AU  - Gatta L
FAU - Marabese, Ida
AU  - Marabese I
FAU - Luongo, Livio
AU  - Luongo L
FAU - Rossi, Claudia
AU  - Rossi C
FAU - de Novellis, Vito
AU  - de Novellis V
FAU - Fernandez-Sanchez, Enrique
AU  - Fernandez-Sanchez E
FAU - Soukupova, Marie
AU  - Soukupova M
FAU - Zafra, Francisco
AU  - Zafra F
FAU - Maione, Sabatino
AU  - Maione S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090923
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Drug Combinations)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 452VLY9402 (Serine)
RN  - H030S2S85J (Kynurenic Acid)
RN  - H9Y79VD43J (Strychnine)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Glycine/*pharmacology
MH  - Kynurenic Acid/pharmacology
MH  - Male
MH  - Medulla Oblongata/*cytology
MH  - Microinjections/methods
MH  - Neural Pathways/physiology
MH  - Neurons/cytology/*physiology
MH  - Pain Measurement/methods
MH  - Pain Threshold/drug effects
MH  - Periaqueductal Gray/*drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Reaction Time/drug effects
MH  - Serine/*pharmacology
MH  - Strychnine/pharmacology
MH  - Tail
EDAT- 2009/09/25 06:00
MHDA- 2010/02/23 06:00
CRDT- 2009/09/25 06:00
PHST- 2009/09/25 06:00 [entrez]
PHST- 2009/09/25 06:00 [pubmed]
PHST- 2010/02/23 06:00 [medline]
AID - 00124.2009 [pii]
AID - 10.1152/jn.00124.2009 [doi]
PST - ppublish
SO  - J Neurophysiol. 2009 Dec;102(6):3169-79. doi: 10.1152/jn.00124.2009. Epub 2009 
      Sep 23.