PMID- 19778335
OWN - NLM
STAT- MEDLINE
DCOM- 20100408
LR  - 20231213
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 50
IP  - 1
DP  - 2010 Jan
TI  - Recent advances in laboratory assays for nonhemolytic transfusion reactions.
PG  - 252-63
LID - 10.1111/j.1537-2995.2009.02388.x [doi]
AB  - Nonhemolytic transfusion reactions (NHTRs) are the most common transfusion 
      reactions and include transfusion-related acute lung injury (TRALI) and allergic 
      and febrile reactions. White blood cell (WBC) antibodies (Abs) against human 
      leukocyte antigen (HLA) and human neutrophil antigen (HNA) in blood components 
      are frequently implicated in NHTRs, especially in TRALI. Recently, we established 
      a five-cell-lineage immunofluorescence test, a modified granulocyte 
      immunofluorescence test, and a panel of cell lines stably expressing HNAs for 
      efficient detection of Abs against well-known HNA-1 to HNA-5, and also Abs 
      against neutrophil antigens other than HNA-1 to HNA-5. Using these techniques, we 
      found that most of the non-HLA WBC Abs detected in NHTR cases were against 
      antigens other than HNA-1 to HNA-5. In addition, using our newly established 
      neutrophil activation test, which assesses neutrophil activation elicited by 
      immunologic stimuli such as WBC Abs and immune complex, we found that 
      heparin-binding protein is a potential final effector molecule that induces 
      NHTRs, including TRALI. More recently, we reported that the basophil activation 
      test, which was originally developed to identify allergens in the field of 
      allergic diseases, might be useful in transfusion medicine. In this review, we 
      summarize these new techniques and other related tests.
FAU - Hirayama, Fumiya
AU  - Hirayama F
AD  - Japanese Red Cross Osaka Blood Center, Morinomiya, Joto-ku, Osaka, Japan. 
      hirayama@osaka.bc.jrc.or.jp
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20090922
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Acute Lung Injury/*diagnosis/etiology
MH  - Basophils/immunology
MH  - Blood Banking/*methods
MH  - Fluorescent Antibody Technique/*methods
MH  - Humans
MH  - Hypersensitivity/*diagnosis/etiology
MH  - Isoantibodies/blood
MH  - Mast Cells/immunology
MH  - Neutrophil Activation
MH  - *Transfusion Reaction
RF  - 88
EDAT- 2009/09/26 06:00
MHDA- 2010/04/09 06:00
CRDT- 2009/09/26 06:00
PHST- 2009/09/26 06:00 [entrez]
PHST- 2009/09/26 06:00 [pubmed]
PHST- 2010/04/09 06:00 [medline]
AID - TRF2388 [pii]
AID - 10.1111/j.1537-2995.2009.02388.x [doi]
PST - ppublish
SO  - Transfusion. 2010 Jan;50(1):252-63. doi: 10.1111/j.1537-2995.2009.02388.x. Epub 
      2009 Sep 22.