PMID- 19779697
OWN - NLM
STAT- MEDLINE
DCOM- 20100420
LR  - 20211020
IS  - 1432-1238 (Electronic)
IS  - 0342-4642 (Linking)
VI  - 35
IP  - 12
DP  - 2009 Dec
TI  - Bronchoalveolar lavage fluid peptidomics suggests a possible matrix 
      metalloproteinase-3 role in bronchopulmonary dysplasia.
PG  - 2115-24
LID - 10.1007/s00134-009-1646-6 [doi]
AB  - BACKGROUND: Bronchoalveolar lavage fluid (BALF) is an important diagnostic source 
      to investigate molecular changes occurring in lung disorders. The objective of 
      this study was to assess and compare the peptidomic profiles of BALF from 
      premature neonates with and without bronchopulmonary dysplasia (BPD). METHODS: 
      Samples were obtained on the 3rd day of life from 34 neonates with gestational 
      age <or=32 weeks. Two pools of samples from patients with and without BPD were 
      analyzed by high performance liquid chromatography. Several differentially 
      expressed peptides were collected and sequenced. Moreover, samples from single 
      donors were analyzed by liquid chromatography-electrospray ionization mass 
      spectrometry to define the molecular mass values of various peptides and to 
      quantify their expression. Levels of some matrix metalloproteinases and their 
      tissue inhibitors were also determined in single samples. RESULTS: Neonates of 
      the BPD group (N = 16) showed significantly lower mean gestational age and birth 
      weight with respect to the no-BPD group (N = 18; P < 0.0001). Levels of six 
      peptides were significantly higher in BPD patients (P < 0.05). Two of them were 
      identified as the albumin fragments 1-21 (2,428 Da) and 399-406 (956 Da). Levels 
      of matrix metalloproteinase-3 (MMP-3) enzyme probably involved in albumin 
      fragment generation were also significantly higher in the BPD group compared to 
      the no-BPD group (P < 0.05), whereas the levels of tissue inhibitor of 
      metalloproteinases-1 were significantly lower (P < 0.05). Levels of albumin 
      fragments and MMP-3 showed a significant correlation (P < 0.05). CONCLUSIONS: 
      This study shows that proteomic techniques can be applied to investigate the 
      involvement of proteolytic enzymes on the airways of mechanically ventilated 
      premature infants.
FAU - Vento, Giovanni
AU  - Vento G
AD  - Division of Neonatology, Department of Pediatrics, School of Medicine, Catholic 
      University, Largo F Vito 1, 00168 Rome, Italy. vento@rm.unicatt.it
FAU - Tirone, Chiara
AU  - Tirone C
FAU - Lulli, Paola
AU  - Lulli P
FAU - Capoluongo, Ettore
AU  - Capoluongo E
FAU - Ameglio, Franco
AU  - Ameglio F
FAU - Lozzi, Simona
AU  - Lozzi S
FAU - Cota, Francesco
AU  - Cota F
FAU - Mosca, Fabio
AU  - Mosca F
FAU - Romagnoli, Costantino
AU  - Romagnoli C
FAU - Messana, Irene
AU  - Messana I
FAU - Castagnola, Massimo
AU  - Castagnola M
FAU - Inzitari, Rosanna
AU  - Inzitari R
LA  - eng
PT  - Journal Article
DEP - 20090925
PL  - United States
TA  - Intensive Care Med
JT  - Intensive care medicine
JID - 7704851
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Bronchoalveolar Lavage Fluid/*chemistry
MH  - Bronchopulmonary Dysplasia/*metabolism/*pathology
MH  - Female
MH  - Gestational Age
MH  - Humans
MH  - Infant, Newborn
MH  - Lung/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 3/*analysis/*metabolism
EDAT- 2009/09/26 06:00
MHDA- 2010/04/21 06:00
CRDT- 2009/09/26 06:00
PHST- 2008/10/13 00:00 [received]
PHST- 2009/08/05 00:00 [accepted]
PHST- 2009/09/26 06:00 [entrez]
PHST- 2009/09/26 06:00 [pubmed]
PHST- 2010/04/21 06:00 [medline]
AID - 10.1007/s00134-009-1646-6 [doi]
PST - ppublish
SO  - Intensive Care Med. 2009 Dec;35(12):2115-24. doi: 10.1007/s00134-009-1646-6. Epub 
      2009 Sep 25.