PMID- 19779711
OWN - NLM
STAT- MEDLINE
DCOM- 20100414
LR  - 20131121
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 85
IP  - 6
DP  - 2010 Feb
TI  - Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin 
      production in Bacillus subtilis.
PG  - 1907-14
LID - 10.1007/s00253-009-2247-6 [doi]
AB  - Carbon flow in Bacillus subtilis through the pentose phosphate (PP) pathway was 
      modulated by overexpression of glucose-6-phosphate dehydrogenase (G6PDH) under 
      the control of the inducible Pxyl promoter in B. subtilis PY. Alteration of 
      carbon flow into the PP pathway will affect the availability of 
      ribulose-5-phosphate (Ru5P) and the riboflavin yield. Overexpression of G6PDH 
      resulted in the glucose consumption rate increasing slightly, while the specific 
      growth rate was unchanged. An improvement by 25% + or - 2 of the riboflavin 
      production was obtained. Compared to by-products formation in flask culture, low 
      acid production (acetate and pyruvate) and more acetoin were observed. Metabolic 
      analysis, together with carbon flux redistribution, indicated that the PP pathway 
      fluxes are increased in response to overexpression of G6PDH. Moreover, increased 
      flux of the PP pathway is associated with an increased intracellular pool of 
      Ru5P, which is a precursor for riboflavin biosynthesis. The high concentrations 
      of Ru5P could explain the increased riboflavin production.
FAU - Duan, Yun Xia
AU  - Duan YX
AD  - Department of Biochemical Engineering, School of Chemical Engineering and 
      Technology, Tianjin University, 92 Weijin Road, Tianjin 300072, China.
FAU - Chen, Tao
AU  - Chen T
FAU - Chen, Xun
AU  - Chen X
FAU - Zhao, Xue Ming
AU  - Zhao XM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090925
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Bacterial Proteins)
RN  - 0 (Pyruvates)
RN  - 0 (Ribulosephosphates)
RN  - 4151-19-3 (ribulose 5-phosphate)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - IY9XDZ35W2 (Glucose)
RN  - TLM2976OFR (Riboflavin)
SB  - IM
MH  - Bacillus subtilis/*enzymology/genetics
MH  - Bacterial Proteins/*biosynthesis/genetics
MH  - *Gene Expression Regulation, Bacterial
MH  - Glucose/metabolism
MH  - Glucosephosphate Dehydrogenase/*biosynthesis/genetics
MH  - Pentose Phosphate Pathway
MH  - Pyruvates/metabolism
MH  - Riboflavin/*biosynthesis/genetics
MH  - Ribulosephosphates/metabolism
EDAT- 2009/09/26 06:00
MHDA- 2010/04/15 06:00
CRDT- 2009/09/26 06:00
PHST- 2009/06/11 00:00 [received]
PHST- 2009/09/06 00:00 [accepted]
PHST- 2009/09/04 00:00 [revised]
PHST- 2009/09/26 06:00 [entrez]
PHST- 2009/09/26 06:00 [pubmed]
PHST- 2010/04/15 06:00 [medline]
AID - 10.1007/s00253-009-2247-6 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2010 Feb;85(6):1907-14. doi: 
      10.1007/s00253-009-2247-6. Epub 2009 Sep 25.