PMID- 19780201
OWN - NLM
STAT- MEDLINE
DCOM- 20100308
LR  - 20220317
IS  - 1098-1136 (Electronic)
IS  - 0894-1491 (Linking)
VI  - 58
IP  - 3
DP  - 2010 Feb
TI  - Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their 
      inhibitors in astrocytes.
PG  - 344-66
LID - 10.1002/glia.20927 [doi]
AB  - Astrocytes play an active role in the central nervous system and are critically 
      involved in astrogliosis, a homotypic response of these cells to disease, injury, 
      and associated neuroinflammation. Among the numerous molecules involved in these 
      processes are the matrix metalloproteinases (MMPs), a family of zinc-dependent 
      endopeptidases, secreted or membrane-bound, that regulate by proteolytic cleavage 
      the extracellular matrix, cytokines, chemokines, cell adhesion molecules, and 
      plasma membrane receptors. MMP activity is tightly regulated by the tissue 
      inhibitors of MMPs (TIMPs), a family of secreted multifunctional proteins. 
      Astrogliosis in vivo and astrocyte reactivity induced in vitro by proinflammatory 
      cues are associated with modulation of expression and/or activity of members of 
      the MMP/TIMP system. However, nothing is known concerning the intracellular 
      distribution and secretory pathways of MMPs and TIMPs in astrocytes. Using a 
      combination of cell biology, biochemistry, fluorescence and electron microscopy 
      approaches, we investigated in cultured reactive astrocytes the intracellular 
      distribution, transport, and secretion of MMP-2, MMP-9, TIMP-1, and TIMP-2. MMP-2 
      and MMP-9 demonstrate nuclear localization, differential intracellular vesicular 
      distribution relative to the myosin V and kinesin molecular motors, and 
      LAMP-2-labeled lysosomal compartment, and we show vesicular secretion for MMP-2, 
      MMP-9, and their inhibitors. Our results suggest that these proteinases and their 
      inhibitors use different pathways for trafficking and secretion for distinct 
      astrocytic functions.
CI  - (c) 2009 Wiley-Liss, Inc.
FAU - Sbai, Oualid
AU  - Sbai O
AD  - Neurobiologie des Interactions Cellulaires et Neurophysiopathologie, UMR 6184 
      CNRS--Universite de la Mediterranee, Faculte de Medecine, 51 Boulevard Pierre 
      Dramard, Marseille Cedex 15, France.
FAU - Ould-Yahoui, Adlane
AU  - Ould-Yahoui A
FAU - Ferhat, Lotfi
AU  - Ferhat L
FAU - Gueye, Yatma
AU  - Gueye Y
FAU - Bernard, Anne
AU  - Bernard A
FAU - Charrat, Eliane
AU  - Charrat E
FAU - Mehanna, Ali
AU  - Mehanna A
FAU - Risso, Jean-Jacques
AU  - Risso JJ
FAU - Chauvin, Jean-Paul
AU  - Chauvin JP
FAU - Fenouillet, Emmanuel
AU  - Fenouillet E
FAU - Rivera, Santiago
AU  - Rivera S
FAU - Khrestchatisky, Michel
AU  - Khrestchatisky M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Lysosomal-Associated Membrane Protein 2)
RN  - 0 (Molecular Motor Proteins)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Active Transport, Cell Nucleus/physiology
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/*enzymology/ultrastructure
MH  - Cell Compartmentation/physiology
MH  - Cells, Cultured
MH  - Encephalitis/*enzymology/physiopathology
MH  - Gliosis/*enzymology/physiopathology
MH  - Lysosomal-Associated Membrane Protein 2/metabolism
MH  - Lysosomes/metabolism
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Mice
MH  - Molecular Motor Proteins/metabolism
MH  - Protein Transport/physiology
MH  - Signal Transduction/physiology
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/metabolism
MH  - Transport Vesicles/*enzymology/ultrastructure
EDAT- 2009/09/26 06:00
MHDA- 2010/03/10 06:00
CRDT- 2009/09/26 06:00
PHST- 2009/09/26 06:00 [entrez]
PHST- 2009/09/26 06:00 [pubmed]
PHST- 2010/03/10 06:00 [medline]
AID - 10.1002/glia.20927 [doi]
PST - ppublish
SO  - Glia. 2010 Feb;58(3):344-66. doi: 10.1002/glia.20927.