PMID- 1978071
OWN - NLM
STAT- MEDLINE
DCOM- 19901221
LR  - 20190610
IS  - 0140-6736 (Print)
IS  - 0140-6736 (Linking)
VI  - 336
IP  - 8725
DP  - 1990 Nov 17
TI  - Antibody profile of early HTLV-I infection.
PG  - 1214-6
AB  - To define the antibody profile of early seroconversion in infection with human 
      T-cell lymphotropic virus type I (HTLV-I), consecutive serum samples from 10 
      subjects presumed to have seroconverted on the basis of the particle 
      agglutination test were studied by three enzyme immunoassays and two confirmatory 
      tests (radioimmunoprecipitation and western blot). 3 samples positive and 1 
      sample indeterminate in the confirmatory tests were reactive in one enzyme 
      immunoassay, which used recombinant envelope antigen, but not in the other two 
      enzyme immunoassays. 2 of 38 particle-agglutination-negative samples had a 
      prozone effect. The confirmatory tests identified 8 seroconverters (7 women, 1 
      man); their serum samples were used to study the antibody reactivity by western 
      blot assays to HTLV-I specific antigens (three recombinant proteins spanning the 
      N-terminal, middle, and C-terminal env glycoprotein gp46; a recombinant 
      transmembrane protein gp21; a recombinant tax protein; and three gag proteins 
      [p28, p24, and p19]). All 8 seroconverters had antibody reactivities to the 
      C-terminal region (aminoacid residues 229-308) of gp46 and to gag p19 and p24 
      when their seroconversion was detected.
FAU - Chen, Y M
AU  - Chen YM
AD  - Department of Cancer Biology, Harvard School of Public Health, Boston, 
      Massachusetts 02115.
FAU - Gomez-Lucia, E
AU  - Gomez-Lucia E
FAU - Okayama, A
AU  - Okayama A
FAU - Tachibana, N
AU  - Tachibana N
FAU - Lee, T H
AU  - Lee TH
FAU - Mueller, N
AU  - Mueller N
FAU - Essex, M
AU  - Essex M
LA  - eng
GR  - 1-P50-HL-33774/HL/NHLBI NIH HHS/United States
GR  - CA 38450/CA/NCI NIH HHS/United States
GR  - HL 6356/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Lancet
JT  - Lancet (London, England)
JID - 2985213R
RN  - 0 (HTLV-I Antibodies)
SB  - IM
EIN - Lancet 1990 Dec 22-29;336(8730):1596
CIN - Lancet. 1991 Jan 19;337(8734):181-2. PMID: 1670825
MH  - Adult
MH  - Aged
MH  - Blotting, Western
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HTLV-I Antibodies/*blood
MH  - HTLV-I Infections/*blood
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Radioimmunoprecipitation Assay
EDAT- 1990/11/17 00:00
MHDA- 1990/11/17 00:01
CRDT- 1990/11/17 00:00
PHST- 1990/11/17 00:00 [pubmed]
PHST- 1990/11/17 00:01 [medline]
PHST- 1990/11/17 00:00 [entrez]
AID - 0140-6736(90)92833-4 [pii]
AID - 10.1016/0140-6736(90)92833-4 [doi]
PST - ppublish
SO  - Lancet. 1990 Nov 17;336(8725):1214-6. doi: 10.1016/0140-6736(90)92833-4.