PMID- 19781942
OWN - NLM
STAT- MEDLINE
DCOM- 20100308
LR  - 20231105
IS  - 1879-0445 (Electronic)
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 19
IP  - 19
DP  - 2009 Oct 13
TI  - The CIL-1 PI 5-phosphatase localizes TRP Polycystins to cilia and activates sperm 
      in C. elegans.
PG  - 1599-607
LID - 10.1016/j.cub.2009.08.045 [doi]
AB  - BACKGROUND: C. elegans male sexual behaviors include chemotaxis and response to 
      hermaphrodites, backing, turning, vulva location, spicule insertion, and sperm 
      transfer, culminating in cross-fertilization of hermaphrodite oocytes with male 
      sperm. The LOV-1 and PKD-2 transient receptor potential polycystin (TRPP) complex 
      localizes to ciliated endings of C. elegans male-specific sensory neurons and 
      mediates several aspects of male mating behavior. TRPP complex ciliary 
      localization and sensory function are evolutionarily conserved. A genetic screen 
      for C. elegans mutants with PKD-2 ciliary localization (Cil) defects led to the 
      isolation of a mutation in the cil-1 gene. RESULTS: Here, we report that a 
      phosphoinositide (PI) 5-phosphatase, CIL-1, regulates TRPP complex ciliary 
      receptor localization and sperm activation. cil-1 does not regulate the 
      localization of other ciliary proteins, including intraflagellar transport (IFT) 
      components, sensory receptors, or other TRP channels in different cell types. 
      Rather, cil-1 specifically controls TRPP complex trafficking in male-specific 
      sensory neurons and does so in a cell-autonomous fashion. In these cells, cil-1 
      is required for normal PI(3)P distribution, indicating that a balance between 
      PI(3,5)P2 and PI(3)P is important for TRPP localization. cil-1 mutants are 
      infertile because of sperm activation and motility defects. In sperm, the CIL-1 
      5-phosphatase and a wortmannin-sensitive PI 3-kinase act antagonistically to 
      regulate the conversion of sessile spermatids into motile spermatozoa, 
      implicating PI(3,4,5)P3 signaling in nematode sperm activation. CONCLUSION: Our 
      studies identify the CIL-1 5-phosphatase as a key regulator of PI metabolism in 
      cell types that are important in several aspects of male reproductive biology.
FAU - Bae, Young-Kyung
AU  - Bae YK
AD  - Department of Genetics, Rutgers University, the State University of New Jersey, 
      Piscataway, NJ 08854, USA.
FAU - Kim, Eunsoo
AU  - Kim E
FAU - L'hernault, Steven W
AU  - L'hernault SW
FAU - Barr, Maureen M
AU  - Barr MM
LA  - eng
GR  - DK074746/DK/NIDDK NIH HHS/United States
GR  - R01 GM082932-03/GM/NIGMS NIH HHS/United States
GR  - R01 DK059418-09/DK/NIDDK NIH HHS/United States
GR  - R01 DK074746-03/DK/NIDDK NIH HHS/United States
GR  - R01 DK059418-06A1/DK/NIDDK NIH HHS/United States
GR  - R01 DK074746-04/DK/NIDDK NIH HHS/United States
GR  - R01 DK059418-07/DK/NIDDK NIH HHS/United States
GR  - DK059418/DK/NIDDK NIH HHS/United States
GR  - R56 DK074746/DK/NIDDK NIH HHS/United States
GR  - R01 GM082932/GM/NIGMS NIH HHS/United States
GR  - R01 DK059418-08/DK/NIDDK NIH HHS/United States
GR  - R01 DK074746/DK/NIDDK NIH HHS/United States
GR  - R01 DK059418/DK/NIDDK NIH HHS/United States
GR  - R01 DK059418-10/DK/NIDDK NIH HHS/United States
GR  - GM082932/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090924
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (LOV-1 protein, C elegans)
RN  - 0 (PKD-2 protein, C elegans)
RN  - 0 (TRPP Cation Channels)
RN  - 0 (Transient Receptor Potential Channels)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.36 (phosphoinositide 5-phosphatase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Caenorhabditis elegans/*enzymology/genetics
MH  - Caenorhabditis elegans Proteins/*genetics/*metabolism
MH  - Cluster Analysis
MH  - DNA Primers/genetics
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Phosphoric Monoester Hydrolases/genetics/*metabolism
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - Sexual Behavior, Animal/*physiology
MH  - Spermatozoa/metabolism/*physiology
MH  - TRPP Cation Channels/*metabolism
MH  - Transient Receptor Potential Channels/*metabolism
PMC - PMC2762383
MID - NIHMS143271
EDAT- 2009/09/29 06:00
MHDA- 2010/03/10 06:00
PMCR- 2010/10/13
CRDT- 2009/09/29 06:00
PHST- 2009/06/01 00:00 [received]
PHST- 2009/08/14 00:00 [revised]
PHST- 2009/08/17 00:00 [accepted]
PHST- 2009/09/29 06:00 [entrez]
PHST- 2009/09/29 06:00 [pubmed]
PHST- 2010/03/10 06:00 [medline]
PHST- 2010/10/13 00:00 [pmc-release]
AID - S0960-9822(09)01621-2 [pii]
AID - 10.1016/j.cub.2009.08.045 [doi]
PST - ppublish
SO  - Curr Biol. 2009 Oct 13;19(19):1599-607. doi: 10.1016/j.cub.2009.08.045. Epub 2009 
      Sep 24.