PMID- 19782698
OWN - NLM
STAT- MEDLINE
DCOM- 20110506
LR  - 20100316
IS  - 1878-5646 (Electronic)
IS  - 0042-6989 (Linking)
VI  - 50
IP  - 7
DP  - 2010 Mar 31
TI  - Transplantation of quantum dot-labelled bone marrow-derived stem cells into the 
      vitreous of mice with laser-induced retinal injury: survival, integration and 
      differentiation.
PG  - 665-73
LID - 10.1016/j.visres.2009.09.003 [doi]
AB  - Accidental laser exposure to the eyes may result in serious visual impairment due 
      to retina degeneration. Currently limited treatment is available for laser eye 
      injury. In the current study, we investigated the therapeutic potential of bone 
      marrow-derived stem cells (BMSCs) for laser-induced retinal trauma. Lineage 
      negative bone marrow cells (Lin(-) BMCs) were labelled with quantum dots (Qdots) 
      to track the cells in vivo. Lin(-) BMCs survived well after intravitreal 
      injection. In vivo bromodeoxyuridine (BrdU) labelling showed these cells 
      continued to proliferate and integrate into injured retinas. Furthermore, they 
      expressed markers that distinguished retinal pigment epithelium (RPE), 
      endothelium, pericytes and photoreceptors. Our results suggest that BMSCs 
      participate in the repair of retinal lesions by differentiating into retinal 
      cells. Intravitreal transplantation of BMSCs is a potential treatment for 
      laser-induced retinal trauma.
CI  - Published by Elsevier Ltd.
FAU - Wang, Heuy-Ching
AU  - Wang HC
AD  - US Army Medical Research Detachment, Walter Reed Army Institute of Research, 7965 
      Dave Erwin Drive, Brooks City-Base, TX 78235, USA. 
      Heuy-ching.hetty.wang@us.army.mil
FAU - Brown, Jeremiah
AU  - Brown J
FAU - Alayon, Helena
AU  - Alayon H
FAU - Stuck, Bruce E
AU  - Stuck BE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090925
PL  - England
TA  - Vision Res
JT  - Vision research
JID - 0417402
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcl12 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Cell Movement/physiology
MH  - Cell Proliferation
MH  - Cell Survival/physiology
MH  - Chemokine CXCL12/metabolism
MH  - Disease Models, Animal
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Immunohistochemistry
MH  - Intravitreal Injections
MH  - Lasers/*adverse effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Polymerase Chain Reaction
MH  - Quantum Dots
MH  - Retina/*cytology/injuries/*surgery
MH  - Vitreous Body/*surgery
EDAT- 2009/09/29 06:00
MHDA- 2011/05/07 06:00
CRDT- 2009/09/29 06:00
PHST- 2009/06/15 00:00 [received]
PHST- 2009/08/31 00:00 [revised]
PHST- 2009/09/03 00:00 [accepted]
PHST- 2009/09/29 06:00 [entrez]
PHST- 2009/09/29 06:00 [pubmed]
PHST- 2011/05/07 06:00 [medline]
AID - S0042-6989(09)00407-6 [pii]
AID - 10.1016/j.visres.2009.09.003 [doi]
PST - ppublish
SO  - Vision Res. 2010 Mar 31;50(7):665-73. doi: 10.1016/j.visres.2009.09.003. Epub 
      2009 Sep 25.