PMID- 19783749
OWN - NLM
STAT- MEDLINE
DCOM- 20100325
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 75
IP  - 22
DP  - 2009 Nov
TI  - Effect of carbohydrate composition in barley and oat cultivars on microbial 
      ecophysiology and proliferation of Salmonella enterica in an in vitro model of 
      the porcine gastrointestinal tract.
PG  - 7006-16
LID - 10.1128/AEM.01343-09 [doi]
AB  - The influence of the carbohydrate (CHO) composition of cereal cultivars on 
      microbial ecophysiology was studied using an in vitro model of the porcine 
      gastrointestinal tract. Ten hull-less barley cultivars, six barley cultivars with 
      hulls, six oat cultivars, and six oat groats that differed in beta-glucan, 
      nonstarch polysaccharide (NSP), and starch contents and starch type were 
      hydrolyzed enzymatically and incubated for 72 h with pig feces. Fermentation 
      kinetics were modeled, and microbial compositions and short-chain fatty acid 
      (SCFA) profiles were analyzed using terminal restriction fragment length 
      polymorphism and gas chromatography. Cluster analysis and canonical ordination 
      revealed different effects on fermentation and microbial ecology depending on the 
      type of CHO and cultivar. First, in cultivars of barley with hulls and oats, the 
      cellulose and insoluble NSP contents (i) increased Ruminococcus flavefaciens-like 
      and Clostridium xylanolyticum-like phylotypes, (ii) increased acetate production, 
      and (iii) decreased fermentation activity. Second, in hull-less barley cultivars 
      the beta-glucan, amylose, amylopectin, crude protein, and soluble NSP contents 
      determined the microbial community composition and activity as follows: (i) the 
      amylose contents of the hull-less barley varieties increased the butyrate 
      production and the abundance of Clostridium butyricum-like phylotypes, (ii) the 
      beta-glucan content determined the total amounts of SCFA, and (iii) the 
      amylopectin and starch contents affected the abundance of Clostridium 
      ramosum-like phylotypes, members of Clostridium cluster XIVa, and 
      Bacteroides-like bacteria. Finally, the effect of CHO on proliferation of 
      Salmonella enterica in the model was determined. Salmonella cell counts were not 
      affected, but the relative proportion of Salmonella decreased with hull-less 
      barley cultivars and increased with oat cultivars as revealed by quantitative 
      PCR. Our results shed light on the complex interactions of cereal CHO with 
      intestinal bacterial ecophysiology and the possible impact on host health.
FAU - Pieper, Robert
AU  - Pieper R
AD  - Department of Poultry and Animal Science, University of Saskatchewan, 51 Campus 
      Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.
FAU - Bindelle, Jerome
AU  - Bindelle J
FAU - Rossnagel, Brian
AU  - Rossnagel B
FAU - Van Kessel, Andrew
AU  - Van Kessel A
FAU - Leterme, Pascal
AU  - Leterme P
LA  - eng
SI  - GENBANK/GQ214260
SI  - GENBANK/GQ214261
SI  - GENBANK/GQ214262
SI  - GENBANK/GQ214263
SI  - GENBANK/GQ214264
SI  - GENBANK/GQ214265
SI  - GENBANK/GQ214266
SI  - GENBANK/GQ214267
SI  - GENBANK/GQ214268
SI  - GENBANK/GQ214269
SI  - GENBANK/GQ214270
SI  - GENBANK/GQ214271
SI  - GENBANK/GQ214272
SI  - GENBANK/GQ214273
SI  - GENBANK/GQ214274
SI  - GENBANK/GQ214275
SI  - GENBANK/GQ214276
SI  - GENBANK/GQ214277
SI  - GENBANK/GQ214278
SI  - GENBANK/GQ214279
SI  - GENBANK/GQ214280
SI  - GENBANK/GQ214281
SI  - GENBANK/GQ214282
SI  - GENBANK/GQ214283
SI  - GENBANK/GQ214284
SI  - GENBANK/GQ214285
SI  - GENBANK/GQ214286
SI  - GENBANK/GQ214287
SI  - GENBANK/GQ214288
SI  - GENBANK/GQ214289
SI  - GENBANK/GQ214290
SI  - GENBANK/GQ214291
SI  - GENBANK/GQ214292
SI  - GENBANK/GQ214293
SI  - GENBANK/GQ214294
SI  - GENBANK/GQ214295
SI  - GENBANK/GQ214296
SI  - GENBANK/GQ214297
SI  - GENBANK/GQ214298
SI  - GENBANK/GQ214299
SI  - GENBANK/GQ214300
SI  - GENBANK/GQ214301
SI  - GENBANK/GQ214302
SI  - GENBANK/GQ214303
SI  - GENBANK/GQ214304
SI  - GENBANK/GQ214305
SI  - GENBANK/GQ214306
SI  - GENBANK/GQ214307
SI  - GENBANK/GQ214308
SI  - GENBANK/GQ214309
SI  - GENBANK/GQ214310
SI  - GENBANK/GQ214311
SI  - GENBANK/GQ214312
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090925
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - *Avena/chemistry/metabolism
MH  - Bacteria/classification/genetics/growth & development/metabolism
MH  - *Bacterial Physiological Phenomena
MH  - Cell Proliferation
MH  - Colony Count, Microbial
MH  - Dietary Carbohydrates/analysis/*metabolism
MH  - Fermentation
MH  - Gastrointestinal Tract/*microbiology
MH  - *Hordeum/chemistry/metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/analysis/metabolism
MH  - Salmonella enterica/growth & development/metabolism/*physiology
MH  - Swine/*microbiology
PMC - PMC2786517
EDAT- 2009/09/29 06:00
MHDA- 2010/03/26 06:00
PMCR- 2010/05/01
CRDT- 2009/09/29 06:00
PHST- 2009/09/29 06:00 [entrez]
PHST- 2009/09/29 06:00 [pubmed]
PHST- 2010/03/26 06:00 [medline]
PHST- 2010/05/01 00:00 [pmc-release]
AID - AEM.01343-09 [pii]
AID - 1343-09 [pii]
AID - 10.1128/AEM.01343-09 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2009 Nov;75(22):7006-16. doi: 10.1128/AEM.01343-09. Epub 
      2009 Sep 25.