PMID- 19784925
OWN - NLM
STAT- MEDLINE
DCOM- 20091123
LR  - 20190227
IS  - 1618-1905 (Electronic)
IS  - 1139-6709 (Linking)
VI  - 12
IP  - 3
DP  - 2009 Sep
TI  - NAD+-dependent post-translational modification of Escherichia coli 
      glyceraldehyde-3-phosphate dehydrogenase.
PG  - 187-92
AB  - Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional 
      housekeeping protein reported to be a target of several covalent modifications in 
      many organisms. In a previous study, enterohemorrhagic (EHEC) and 
      enteropathogenic (EPEC) Escherichia coli strains were shown to secrete GAPDH and 
      the protein to bind human plasminogen and fibrinogen. Here we report that GAPDH 
      of these pathogens is ADP-ribosylated either in the cytoplasm or in the 
      extracellular medium. GAPDH catalyzes its own modification, which involves 
      Cys-149 at the active site. ADP-ribosylation of extracellular GAPDH may play an 
      important role in the host-pathogen interaction, as also proposed in other 
      pathogens.
FAU - Aguilera, Laura
AU  - Aguilera L
AD  - Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the 
      University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, 
      Barcelona, Spain.
FAU - Gimenez, Rosa
AU  - Gimenez R
FAU - Badia, Josefa
AU  - Badia J
FAU - Aguilar, Juan
AU  - Aguilar J
FAU - Baldoma, Laura
AU  - Baldoma L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Int Microbiol
JT  - International microbiology : the official journal of the Spanish Society for 
      Microbiology
JID - 9816585
RN  - 20762-30-5 (Adenosine Diphosphate Ribose)
RN  - EC 1.2.1.12 (Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating))
SB  - IM
MH  - Adenosine Diphosphate Ribose/metabolism
MH  - Amino Acid Sequence
MH  - Enterohemorrhagic Escherichia coli/*enzymology/*metabolism
MH  - Enteropathogenic Escherichia coli/*enzymology/*metabolism
MH  - Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Protein Processing, Post-Translational
EDAT- 2009/09/29 06:00
MHDA- 2009/12/16 06:00
CRDT- 2009/09/29 06:00
PHST- 2009/09/29 06:00 [entrez]
PHST- 2009/09/29 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - im2306137 [pii]
PST - ppublish
SO  - Int Microbiol. 2009 Sep;12(3):187-92.