PMID- 19787145
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20190606
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Linking)
VI  - 42
IP  - 10
DP  - 2009 Oct
TI  - High expression of the circadian gene mPer2 diminishes the radiosensitivity of 
      NIH 3T3 cells.
PG  - 882-91
LID - S0100-879X2009005000022 [pii]
AB  - Period2 is a core circadian gene, which not only maintains the circadian rhythm 
      of cells but also regulates some organic functions. We investigated the effects 
      of mPeriod2 (mPer2) expression on radiosensitivity in normal mouse cells exposed 
      to 60Co-gamma-rays. NIH 3T3 cells were treated with 
      12-O-tetradecanoylphorbol-13-acetate (TPA) to induce endogenous mPer2 expression 
      or transfected with pcDNA3.1(+)-mPer2 and irradiated with 60Co-gamma-rays, and 
      then analyzed by several methods such as flow cytometry, colony formation assay, 
      RT-PCR, and immunohistochemistry. Flow cytometry and colony formation assay 
      revealed that irradiated NIH 3T3 cells expressing high levels of mPer2 showed a 
      lower death rate (TPA: 24 h 4.3% vs 12 h 6.8% and control 9.4%; transfection: 
      pcDNA3.1-mPer2 3.7% vs pcDNA3.1 11.3% and control 8.2%), more proliferation and 
      clonogenic survival (TPA: 121.7 +/- 6.51 vs 66.0 +/- 3.51 and 67.7 +/- 7.37; 
      transfection: 121.7 +/- 6.50 vs 65.3 +/- 3.51 and 69.0 +/- 4.58) both when 
      treated with TPA and transfected with mPer2. RT-PCR analysis showed an increased 
      expression of bax, bcl-2, p53, c-myc, mre11, and nbs1, and an increased 
      proportionality of bcl-2/bax in the irradiated cells at peak mPer2 expression 
      compared with cells at trough mPer2 expression and control cells. However, no 
      significant difference in rad50 expression was observed among the three groups of 
      cells. Immunohistochemistry also showed increased protein levels of P53, BAX and 
      proliferating cell nuclear antigen in irradiated cells with peak mPer2 levels. 
      Thus, high expression of the circadian gene mPer2 may reduce the radiosensitivity 
      of NIH 3T3 cells. For this effect, mPer2 may directly or indirectly regulate the 
      expressions of cell proliferation- and apoptosis-related genes and DNA 
      repair-related genes.
FAU - Chang, L
AU  - Chang L
AD  - Health Ministry Key Laboratory of Chronobiology, West China Medical Center, 
      Sichuan University, Chengdu, China.
FAU - Liu, Y Y
AU  - Liu YY
FAU - Zhu, B
AU  - Zhu B
FAU - Li, Y
AU  - Li Y
FAU - Hua, H
AU  - Hua H
FAU - Wang, Y H
AU  - Wang YH
FAU - Zhang, J
AU  - Zhang J
FAU - Jiang, Z
AU  - Jiang Z
FAU - Wang, Z R
AU  - Wang ZR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090904
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Per2 protein, mouse)
RN  - 0 (Period Circadian Proteins)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Apoptosis/*radiation effects
MH  - Mice
MH  - NIH 3T3 Cells/drug effects/radiation effects
MH  - Period Circadian Proteins/*genetics/radiation effects
MH  - Radiation Tolerance
MH  - Tetradecanoylphorbol Acetate
MH  - Transfection
EDAT- 2009/09/30 06:00
MHDA- 2010/05/07 06:00
CRDT- 2009/09/30 06:00
PHST- 2009/01/23 00:00 [received]
PHST- 2009/07/22 00:00 [accepted]
PHST- 2009/09/30 06:00 [entrez]
PHST- 2009/09/30 06:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
AID - S0100-879X2009005000022 [pii]
AID - 10.1590/s0100-879x2009005000022 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2009 Oct;42(10):882-91. doi: 
      10.1590/s0100-879x2009005000022. Epub 2009 Sep 4.