PMID- 19787423
OWN - NLM
STAT- MEDLINE
DCOM- 20100402
LR  - 20231214
IS  - 1880-6562 (Electronic)
IS  - 1880-6546 (Print)
IS  - 1880-6546 (Linking)
VI  - 60
IP  - 1
DP  - 2010 Jan
TI  - Light-induced COP9 signalosome expression in the Indian false vampire bat 
      Megaderma lyra.
PG  - 43-9
LID - 10.1007/s12576-009-0064-4 [doi]
AB  - The COP9 signalosome (CSN) is a multi-subunit protein complex conserved in plants 
      and animals. CSN subunits have been identified as light-mediated master 
      regulators of eukaryotic circadian clocks from fungi to animals. The Indian false 
      vampire bat Megaderma lyra is completely adapted to an anthropic biotope and 
      behavioral studies have reported that M. lyra exhibits light-sampling behavior to 
      assess environmental light. LC-MS-MS results for a 36 kDa protein were analyzed 
      using the Sequest search engine, and COP9 signalosome subunit 5 (CSN5) was 
      pinpointed as having the highest score with 6 matching peptides. To confirm the 
      presence of CSN5, up-regulated cDNA was amplified, sequenced, and identified as 
      CSN5. Furthermore, semi-quantitative RT-PCR analysis demonstrated that the level 
      of induction of CSN5 was regulated by environmental light. We estimated the level 
      of expression across a light-dark cycle and observed a higher level of expression 
      at the end of the light phase. Similarly, when the animal was shifted from 
      continuous dark to light, CSN5 expression was induced. Correspondingly, we 
      detected the similar pattern of translated protein with JAB1 antibody. Knowledge 
      about the circadian rhythm and its molecular mechanism in Chiroptera is very 
      limited and this study suggests that CSN5 might be involved in the M. lyra 
      light-signaling process.
FAU - Rajan, K Emmanuvel
AU  - Rajan KE
AD  - Department of Animal Science, School of Life Sciences, Bharathidasan University, 
      Tiruchirappalli 620024, India. emmanuvel1972@yahoo.com
FAU - Rajkumar, R
AU  - Rajkumar R
FAU - Liao, Chen-Chug
AU  - Liao CC
FAU - Ganesh, A
AU  - Ganesh A
FAU - Marimuthu, G
AU  - Marimuthu G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090929
PL  - Japan
TA  - J Physiol Sci
JT  - The journal of physiological sciences : JPS
JID - 101262417
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Protein Subunits)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Brain Chemistry
MH  - Cell Nucleus/*metabolism/*radiation effects
MH  - Chiroptera/*physiology
MH  - Intracellular Signaling Peptides and Proteins/*metabolism/radiation effects
MH  - *Light
MH  - Nuclear Proteins/*genetics/radiation effects
MH  - Protein Subunits/genetics
MH  - Signal Transduction/genetics
PMC - PMC10717346
EDAT- 2009/09/30 06:00
MHDA- 2010/04/03 06:00
PMCR- 2009/09/29
CRDT- 2009/09/30 06:00
PHST- 2009/05/05 00:00 [received]
PHST- 2009/09/09 00:00 [accepted]
PHST- 2009/09/30 06:00 [entrez]
PHST- 2009/09/30 06:00 [pubmed]
PHST- 2010/04/03 06:00 [medline]
PHST- 2009/09/29 00:00 [pmc-release]
AID - 64 [pii]
AID - 10.1007/s12576-009-0064-4 [doi]
PST - ppublish
SO  - J Physiol Sci. 2010 Jan;60(1):43-9. doi: 10.1007/s12576-009-0064-4. Epub 2009 Sep 
      29.