PMID- 19787603
OWN - NLM
STAT- MEDLINE
DCOM- 20091103
LR  - 20220310
IS  - 1120-6721 (Print)
IS  - 1120-6721 (Linking)
VI  - 19
IP  - 5
DP  - 2009 Sep-Oct
TI  - Intravitreal infliximab in experimental endotoxin-induced uveitis.
PG  - 818-23
LID - 7C3B4E16-FDB6-4A88-B9C7-AA3055D2095F [pii]
AB  - PURPOSE: Tumor necrosis factor-alpha (TNF-alpha) plays a central role in the 
      pathogenesis of ocular inflammation and the level of TNF-alpha is increased in 
      ocular fluids of patients with uveitis. Intravenous infliximab, a monoclonal 
      antibody against TNF-alpha, has been used for the treatment of uveitis with 
      promising preliminary results. The aim of this study was to evaluate the effect 
      of intravitreal injection of infliximab on experimental uveitis. METHODS: 
      Thirty-three white New Zealand rabbits were divided randomly into three groups. 
      Group 1 (n=5) received intravitreal injection of 1 mg/0.1 cc infliximab plus 0.1 
      cc normal saline, group 2 (n=14) received intravitreal injection of 2 microg 
      Salmonella typhimurium endotoxin plus 1 mg/0.1 cc infliximab, and group 3 (n=14) 
      animals received intravitreal endotoxin 2 microg/0.1 cc plus normal saline 0.1 
      cc. Inflammation was evaluated by clinical examinations on days 1, 3, 5, and 7 
      after the injections; measuring the protein concentration and inflammatory cell 
      content of the aqueous humor; and histopathologic examination. RESULTS: No 
      inflammation occurred in group 1 animals. There was a statistically significant 
      difference between group 2 and 3 animals with regard to clinical examination on 
      the third, fifth, and seventh postinjection days. The differences between groups 
      2 and 3 were significant with regard to aqueous cell counts and protein content 
      at day 7 (p=0.02 and p=0.001, respectively). Histopathologic examination results 
      showed less inflammation in group 2 animals compared to group 3 animals 
      (p=0.009). CONCLUSIONS: The results provide evidence that intravitreal injection 
      of infliximab suppresses ocular inflammation in a rabbit model of severe 
      endotoxin-induced uveitis.
FAU - Hosseini, Hamid
AU  - Hosseini H
AD  - Department of Ophthalmology, Poostchi Ophthalmic Research Center, Shiraz 
      University of Medical Sciences, Shiraz - Iran. hosseini.hamid@gmail.com
FAU - Safaei, Akbar
AU  - Safaei A
FAU - Khalili, Mohammad R
AU  - Khalili MR
FAU - Nowroozizadeh, Behdokht
AU  - Nowroozizadeh B
FAU - Eghtedari, Masoometh
AU  - Eghtedari M
FAU - Farvardin, Mohsen
AU  - Farvardin M
FAU - Nowroozizadeh, Sarah
AU  - Nowroozizadeh S
FAU - Tolide-Ie, Hamid R
AU  - Tolide-Ie HR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Eur J Ophthalmol
JT  - European journal of ophthalmology
JID - 9110772
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Eye Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - B72HH48FLU (Infliximab)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*administration & dosage
MH  - Antibodies, Monoclonal/*administration & dosage
MH  - Aqueous Humor/cytology/metabolism
MH  - *Disease Models, Animal
MH  - Eye Proteins/metabolism
MH  - Infliximab
MH  - Injections
MH  - Lipopolysaccharides
MH  - Rabbits
MH  - Salmonella typhimurium
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors
MH  - Uveitis/chemically induced/*drug therapy/pathology
MH  - Vitreous Body
EDAT- 2009/09/30 06:00
MHDA- 2009/11/05 06:00
CRDT- 2009/09/30 06:00
PHST- 2009/09/30 06:00 [entrez]
PHST- 2009/09/30 06:00 [pubmed]
PHST- 2009/11/05 06:00 [medline]
AID - 7C3B4E16-FDB6-4A88-B9C7-AA3055D2095F [pii]
AID - 10.1177/112067210901900521 [doi]
PST - ppublish
SO  - Eur J Ophthalmol. 2009 Sep-Oct;19(5):818-23. doi: 10.1177/112067210901900521.