PMID- 19787789
OWN - NLM
STAT- MEDLINE
DCOM- 20100715
LR  - 20181201
IS  - 1097-0347 (Electronic)
IS  - 1043-3074 (Linking)
VI  - 32
IP  - 5
DP  - 2010 May
TI  - Dendritic cells pulsed with GST-EGFR fusion protein: effect in antitumor immunity 
      against head and neck squamous cell carcinoma.
PG  - 626-35
LID - 10.1002/hed.21233 [doi]
AB  - BACKGROUND: Overexpression of epidermal growth factor receptor (EGFR) is common 
      in head and neck squamous cell carcinoma (HNSCC). Targeting EGFR is an effective 
      approach to treat EGFR-positive HNSCC. However, the clinical benefits of the 
      present EGFR-targeting agents are still limited in HNSCC patients. METHODS: 
      Recombinant glutathione-S-transferase (GST)-EGFR fusion protein was produced and 
      purified. Dendritic cells (DCs) of C3H mice were pulsed with fusion protein. Mice 
      were challenged with HNSCC cells before or after vaccination with these DCs, and 
      the cytotoxic T-lymphocyte (CTL) response, interferon-gamma (IFN-gamma) 
      secretion, tumor growth, and survival of mice were assessed. RESULTS: Significant 
      in vitro and in vivo antitumor activities were observed for mice immunized with 
      DCs pulsed with GST-EGFR fusion protein, compared with the control groups (p < 
      .05). CONCLUSION: The DCs pulsed with GST-EGFR fusion protein can provide not 
      only preventive but also therapeutic antitumor activities against HNSCC in the 
      animal model.
FAU - Yang, Bei-Bei
AU  - Yang BB
AD  - Department of Otolaryngology, 2nd Affiliated Hospital, College of Medicine, 
      Zhejiang University, Zhejiang, China.
FAU - Jiang, Hua
AU  - Jiang H
FAU - Chen, Jia
AU  - Chen J
FAU - Zhang, Xing
AU  - Zhang X
FAU - Ye, Jing-Jia
AU  - Ye JJ
FAU - Cao, Jiang
AU  - Cao J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (Cancer Vaccines)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Cancer Vaccines
MH  - Carcinoma, Squamous Cell/*immunology/therapy
MH  - Dendritic Cells/*immunology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - ErbB Receptors
MH  - Female
MH  - Glutathione Transferase/*pharmacology
MH  - Head and Neck Neoplasms/*immunology/therapy
MH  - Interferon-gamma/metabolism
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Recombinant Fusion Proteins/*pharmacology
MH  - T-Lymphocytes/immunology
EDAT- 2009/09/30 06:00
MHDA- 2010/07/16 06:00
CRDT- 2009/09/30 06:00
PHST- 2009/09/30 06:00 [entrez]
PHST- 2009/09/30 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
AID - 10.1002/hed.21233 [doi]
PST - ppublish
SO  - Head Neck. 2010 May;32(5):626-35. doi: 10.1002/hed.21233.