PMID- 19796141
OWN - NLM
STAT- MEDLINE
DCOM- 20100625
LR  - 20100512
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 70
IP  - 3
DP  - 2009 Dec
TI  - Individual hydrothermal vents at Axial Seamount harbor distinct subseafloor 
      microbial communities.
PG  - 413-24
LID - 10.1111/j.1574-6941.2009.00747.x [doi]
AB  - The microbial community structure of five geographically distinct hydrothermal 
      vents located within the Axial Seamount caldera, Juan de Fuca Ridge, was examined 
      over 6 years following the 1998 diking eruptive event. Terminal restriction 
      fragment length polymorphism (TRFLP) and 16S rRNA gene sequence analyses were 
      used to determine the bacterial and archaeal diversity, and the statistical 
      software primer v6 was used to compare vent microbiology, temperature and fluid 
      chemistry. Statistical analysis of vent fluid temperature and composition shows 
      that there are significant differences between vents in any year, but that the 
      fluid composition changes over time such that no vent maintains a chemical 
      composition completely distinct from the others. In contrast, the subseafloor 
      microbial communities associated with individual vents changed from year to year, 
      but each location maintained a distinct community structure (based on TRFLP and 
      16S rRNA gene sequence analyses) that was significantly different from all other 
      vents included in this study. Epsilonproteobacterial microdiversity is shown to 
      be important in distinguishing vent communities, while archaeal microdiversity is 
      less variable between sites. We propose that persistent venting at diffuse flow 
      vents over time creates the potential to isolate and stabilize diverse microbial 
      community structures between vents.
FAU - Opatkiewicz, Andrew D
AU  - Opatkiewicz AD
AD  - School of Oceanography and Center for Astrobiology and Early Evolution, 
      University of Washington, Seattle, WA 98195, USA. opatka@u.washington.edu
FAU - Butterfield, David A
AU  - Butterfield DA
FAU - Baross, John A
AU  - Baross JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090921
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Archaea/classification/genetics/*isolation & purification
MH  - Bacteria/classification/genetics/*isolation & purification
MH  - *Biodiversity
MH  - DNA, Archaeal/genetics
MH  - DNA, Bacterial/genetics
MH  - Geologic Sediments/microbiology
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/chemistry/*microbiology
MH  - Temperature
MH  - *Water Microbiology
EDAT- 2009/10/03 06:00
MHDA- 2010/06/26 06:00
CRDT- 2009/10/03 06:00
PHST- 2009/10/03 06:00 [entrez]
PHST- 2009/10/03 06:00 [pubmed]
PHST- 2010/06/26 06:00 [medline]
AID - FEM747 [pii]
AID - 10.1111/j.1574-6941.2009.00747.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2009 Dec;70(3):413-24. doi: 
      10.1111/j.1574-6941.2009.00747.x. Epub 2009 Sep 21.