PMID- 19796804
OWN - NLM
STAT- MEDLINE
DCOM- 20100105
LR  - 20161125
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 30
IP  - 36
DP  - 2009 Dec
TI  - Enhanced human bone marrow stromal cell affinity for modified poly(L-lactide) 
      surfaces by the upregulation of adhesion molecular genes.
PG  - 6903-11
LID - 10.1016/j.biomaterials.2009.09.013 [doi]
AB  - To enhance and regulate cell affinity for poly (L-lactic acid) (PLLA) based 
      materials, two hydrophilic ligands, poly (ethylene glycol) (PEG) and poly 
      (L-lysine) (PLL), were used to develop triblock copolymers: methoxy-terminated 
      poly (ethylene glycol)-block-poly (L-lactide)-block-poly (L-lysine) 
      (MPEG-b-PLLA-b-PLL) in order to regulate protein absorption and cell adhesion. 
      Bone marrow stromal cells (BMSCs) were cultured on different composition of 
      MPEG-b-PLLA-b-PLL copolymer films to determine the effect of modified polymer 
      surfaces on BMSC attachment. To understand the molecular mechanism governing the 
      initial cell adhesion on difference polymer surfaces, the mRNA expression of 84 
      human extracellular matrix (ECM) and adhesion molecules was analysed using 
      quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). It was 
      found that down regulation of adhesion molecules was responsible for the impaired 
      BMSC attachment on PLLA surface. MPEG-b-PLLA-b-PLL copolymer films improved 
      significantly the cell adhesion and cytoskeleton expression by upregulation of 
      relevant molecule genes significantly. Six adhesion genes (CDH1, ITGL, NCAM1, 
      SGCE, COL16A1, and LAMA3) were most significantly influenced by the modified PLLA 
      surfaces. In summary, polymer surfaces altered adhesion molecule gene expression 
      of BMSCs, which consequently regulated cell initial attachment on modified PLLA 
      surfaces.
FAU - Mao, Xueli
AU  - Mao X
AD  - Institute of Health and Biomedical Innovation, Queensland University of 
      Technology, 60 Musk Ave, Kelvin Grove Campus, Brisbane, QLD 4059, Australia.
FAU - Peng, Hui
AU  - Peng H
FAU - Ling, Junqi
AU  - Ling J
FAU - Friis, Thor
AU  - Friis T
FAU - Whittaker, Andrew K
AU  - Whittaker AK
FAU - Crawford, Ross
AU  - Crawford R
FAU - Xiao, Yin
AU  - Xiao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090930
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 459TN2L5F5 (poly(lactide))
SB  - IM
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Cell Adhesion/*physiology
MH  - Cell Adhesion Molecules/*genetics/metabolism
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Cytoskeleton/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Polyesters/*chemistry
MH  - Polymers/chemistry
MH  - Stromal Cells/cytology/*physiology
MH  - Surface Properties
EDAT- 2009/10/03 06:00
MHDA- 2010/01/06 06:00
CRDT- 2009/10/03 06:00
PHST- 2009/08/03 00:00 [received]
PHST- 2009/09/04 00:00 [accepted]
PHST- 2009/10/03 06:00 [entrez]
PHST- 2009/10/03 06:00 [pubmed]
PHST- 2010/01/06 06:00 [medline]
AID - S0142-9612(09)00929-6 [pii]
AID - 10.1016/j.biomaterials.2009.09.013 [doi]
PST - ppublish
SO  - Biomaterials. 2009 Dec;30(36):6903-11. doi: 10.1016/j.biomaterials.2009.09.013. 
      Epub 2009 Sep 30.