PMID- 19797464
OWN - NLM
STAT- MEDLINE
DCOM- 20100105
LR  - 20220331
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 59
IP  - Pt 1
DP  - 2010 Jan
TI  - Accelerated identification of Staphylococcus aureus from blood cultures by a 
      modified fluorescence in situ hybridization procedure.
PG  - 65-68
LID - 10.1099/jmm.0.015503-0 [doi]
AB  - This study evaluated fluorescence in situ hybridization (FISH) for rapid 
      identification of Staphylococcus aureus and coagulase-negative staphylococci 
      (CoNS) directly from blood cultures. Initially, 360 blood cultures containing 
      Gram-positive cocci were investigated by a previously described microwave-FISH 
      procedure: 44/49 (89.8 %) S. aureus and 298/299 (99.7 %) CoNS were correctly 
      identified. Because FISH proved useful and reliable but handling was found to be 
      inconvenient, the method was modified by employing a recently developed slide 
      chamber. This reduced the time required from 60 to 30 min. The simplified 
      execution allowed integration of the method into the workflow of a routine 
      laboratory without difficulty. The modified method proved to be highly reliable, 
      identifying 37/37 (100 %) S. aureus and 169/172 (98.2 %) CoNS directly from blood 
      cultures.
FAU - Poppert, Sven
AU  - Poppert S
AD  - Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
FAU - Riecker, Melanie
AU  - Riecker M
AD  - Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany.
FAU - Wellinghausen, Nele
AU  - Wellinghausen N
AD  - Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany.
FAU - Frickmann, Hagen
AU  - Frickmann H
AD  - Institute of Medical Microbiology, Virology and Hygiene, University of Rostock, 
      Rostock, Germany.
FAU - Essig, Andreas
AU  - Essig A
AD  - Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
SB  - IM
MH  - Bacteriological Techniques
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microwaves
MH  - Staphylococcal Infections/*blood/*microbiology
MH  - Staphylococcus aureus/*isolation & purification
EDAT- 2009/10/03 06:00
MHDA- 2010/01/06 06:00
CRDT- 2009/10/03 06:00
PHST- 2009/10/03 06:00 [entrez]
PHST- 2009/10/03 06:00 [pubmed]
PHST- 2010/01/06 06:00 [medline]
AID - 10.1099/jmm.0.015503-0 [doi]
PST - ppublish
SO  - J Med Microbiol. 2010 Jan;59(Pt 1):65-68. doi: 10.1099/jmm.0.015503-0.