PMID- 19798096
OWN - NLM
STAT- MEDLINE
DCOM- 20100127
LR  - 20220321
IS  - 1476-5551 (Electronic)
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 24
IP  - 1
DP  - 2010 Jan
TI  - Stem cell traits in long-term co-culture revealed by time-lapse imaging.
PG  - 153-61
LID - 10.1038/leu.2009.191 [doi]
AB  - A deeper understanding of stem cell niche engagement and subsequent behaviors 
      would be enhanced by technologies enabling the tracking of individual stem cells 
      at the clonal level in long-term co-culture (LTC), which mimics the complexity of 
      the bone marrow microenvironment in vivo. Here, we report the application of 
      time-lapse imaging with intermittent fluorescence for tracking well-defined 
      populations of GFP(+) murine hematopoietic stem cells (HSCs) using LTC for >5 
      weeks. Long-term (LT) and short-term (ST) repopulating HSCs and hematopoietic 
      progenitor cells (HPCs) were compared. The transition from cobblestone areas 
      (CAs) under the stromal cell mantle into dispersed migrating cells on top of the 
      stroma (COS) were directly observed. The ST-HSC and LT-HSC were able to initiate 
      multiple waves of CA formation and COS expansion beyond 2 and 4 weeks, 
      respectively. Retrospective tracking of individual CA forming cell (CAFC) 
      revealed a preference for residing under stroma before the first division and a 
      longer interval before first division for LT-HSC. Inability to maintain 
      quiescence in subsequent divisions was revealed. Our study represents an 
      important starting point from which the LTC system can be augmented to provide a 
      better in vitro model for bone marrow stem cell niches.
FAU - Song, Y
AU  - Song Y
AD  - Department of Radiation Oncology, University of Pittsburgh School of Medicine, 
      Cancer Stem Cell Program, University of Pittsburgh Cancer Institute, Pittsburgh, 
      PA, USA.
FAU - Bahnson, A
AU  - Bahnson A
FAU - Hall, N
AU  - Hall N
FAU - Yu, H
AU  - Yu H
FAU - Shen, H
AU  - Shen H
FAU - Koebler, D
AU  - Koebler D
FAU - Houck, R
AU  - Houck R
FAU - Xie, Y
AU  - Xie Y
FAU - Cheng, T
AU  - Cheng T
LA  - eng
GR  - R01 HL070561-06/HL/NHLBI NIH HHS/United States
GR  - R01 HL070561-05A2/HL/NHLBI NIH HHS/United States
GR  - R01 HL 075601/HL/NHLBI NIH HHS/United States
GR  - R01 HL070561-03/HL/NHLBI NIH HHS/United States
GR  - R01 AI080424-01/AI/NIAID NIH HHS/United States
GR  - R01 HL070561-04/HL/NHLBI NIH HHS/United States
GR  - R01 EB 001051/EB/NIBIB NIH HHS/United States
GR  - R01 AI080424/AI/NIAID NIH HHS/United States
GR  - R01 HL070561/HL/NHLBI NIH HHS/United States
GR  - R01 EB001051/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20091001
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - Coculture Techniques
MH  - Hematopoietic Stem Cells/*cytology
MH  - Immunophenotyping
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Fluorescence
MH  - Stromal Cells/cytology
MH  - Time Factors
PMC - PMC2806505
MID - NIHMS139942
EDAT- 2009/10/03 06:00
MHDA- 2010/01/28 06:00
PMCR- 2010/07/01
CRDT- 2009/10/03 06:00
PHST- 2009/10/03 06:00 [entrez]
PHST- 2009/10/03 06:00 [pubmed]
PHST- 2010/01/28 06:00 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - leu2009191 [pii]
AID - 10.1038/leu.2009.191 [doi]
PST - ppublish
SO  - Leukemia. 2010 Jan;24(1):153-61. doi: 10.1038/leu.2009.191. Epub 2009 Oct 1.