PMID- 19798748
OWN - NLM
STAT- MEDLINE
DCOM- 20100325
LR  - 20220310
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 88
IP  - 4
DP  - 2010 Mar
TI  - Enzymatic-nonenzymatic cellular antioxidant defense systems response and 
      immunohistochemical detection of MDMA, VMAT2, HSP70, and apoptosis as biomarkers 
      for MDMA (Ecstasy) neurotoxicity.
PG  - 905-16
LID - 10.1002/jnr.22245 [doi]
AB  - 3,4-Methylenedioxymethamphetamine (MDMA)-induced neurotoxicity leads to the 
      formation of quinone metabolities and hydroxyl radicals and then to the 
      production of reactive oxygen species (ROS). We evaluated the effect of a single 
      dose of MDMA (20 mg/kg, i.p.) on the enzymatic and nonenzymatic cellular 
      antioxidant defense system in different areas of rat brain in the early hours (<6 
      hr) of the administration itself, and we identified the morphological expressions 
      of neurotoxicity induced by MDMA on the vulnerable brain areas in the first 24 
      hr. The acute administration of MDMA produces a decrease of reduced and oxidized 
      glutathione ratio, and antioxidant enzyme activities were significantly reduced 
      after 3 hr and after 6 hr in frontal cortex. Ascorbic acid levels strongly 
      increased in striatum, hippocampus, and frontal cortex after 3 and 6 hr. High 
      levels of malonaldehyde with respect to control were measured in striatum after 3 
      and 6 hr and in hippocampus and frontal cortex after 6 hr. An immunohistochemical 
      investigation on the frontal, thalamic, hypothalamic, and striatal areas was 
      performed. A strong positive reaction to the antivesicular monoamine transporter 
      2 was observed in the frontal section, in the basal ganglia and thalamus. 
      Cortical positivity, located in the most superficial layer was revealed only for 
      heat shock protein 70 after 24 hr.
FAU - Riezzo, Irene
AU  - Riezzo I
AD  - Department of Forensic Pathology, Faculty of Medicine, University of Foggia, 
      Foggia, Italy.
FAU - Cerretani, Daniela
AU  - Cerretani D
FAU - Fiore, Carmela
AU  - Fiore C
FAU - Bello, Stefania
AU  - Bello S
FAU - Centini, Fabio
AU  - Centini F
FAU - D'Errico, Stefano
AU  - D'Errico S
FAU - Fiaschi, Anna Ida
AU  - Fiaschi AI
FAU - Giorgi, Giorgio
AU  - Giorgi G
FAU - Neri, Margherita
AU  - Neri M
FAU - Pomara, Cristoforo
AU  - Pomara C
FAU - Turillazzi, Emanuela
AU  - Turillazzi E
FAU - Fineschi, Vittorio
AU  - Fineschi V
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Biomarkers)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Hallucinogens)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Slc18a2 protein, rat)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - GAN16C9B8O (Glutathione)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Brain/drug effects/metabolism/pathology
MH  - Chromatography, High Pressure Liquid/methods
MH  - Disease Models, Animal
MH  - Electrochemistry/methods
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Glutathione/metabolism
MH  - HSP70 Heat-Shock Proteins/*metabolism
MH  - Hallucinogens/*metabolism/toxicity
MH  - Male
MH  - Malondialdehyde
MH  - N-Methyl-3,4-methylenedioxyamphetamine/metabolism/toxicity
MH  - Neurotoxicity Syndromes/etiology/*metabolism/pathology
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Time Factors
MH  - Vesicular Monoamine Transport Proteins/*metabolism
EDAT- 2009/10/03 06:00
MHDA- 2010/03/26 06:00
CRDT- 2009/10/03 06:00
PHST- 2009/10/03 06:00 [entrez]
PHST- 2009/10/03 06:00 [pubmed]
PHST- 2010/03/26 06:00 [medline]
AID - 10.1002/jnr.22245 [doi]
PST - ppublish
SO  - J Neurosci Res. 2010 Mar;88(4):905-16. doi: 10.1002/jnr.22245.