PMID- 19799176
OWN - NLM
STAT- MEDLINE
DCOM- 20091109
LR  - 20211020
IS  - 1064-3745 (Print)
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 557
DP  - 2009
TI  - Measurement of spatial proximity and accessibility of chromosomal loci in 
      Saccharomyces cerevisiae using Cre/loxP site-specific recombination.
PG  - 55-63
LID - 10.1007/978-1-59745-527-5_5 [doi]
AB  - Several methods have been developed to measure interactions between homologous 
      chromosomes during meiosis in budding yeast. These include cytological analysis 
      of fixed, spread nuclei using fluorescence in situ hybridization (FISH) (1, 2), 
      visualization of GFP-labeled chromosomal loci in living cells (3), and 
      Chromosome-Conformation Capture (3C) (4). Here we describe a quantitative genetic 
      assay that uses exogenous site-specific recombination to monitor the level of 
      homolog associations between two defined loci in living cells of budding yeast 
      (5). We have used the Cre/loxP assay to genetically dissect nuclear architecture 
      and meiotic homolog pairing in budding yeast. Data obtained from this assay 
      report on the relative spatial proximity or accessibility of two chromosomal loci 
      located within the same strain and can be compared to measurements from different 
      mutated strains.
FAU - Lui, Doris
AU  - Lui D
AD  - College of Biological Sciences, University of California, Davis, CA, USA.
FAU - Burgess, Sean M
AU  - Burgess SM
LA  - eng
GR  - R01 GM075119/GM/NIGMS NIH HHS/United States
GR  - R01 GM075119-04/GM/NIGMS NIH HHS/United States
GR  - T 32 ES07059/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Fungal/*chemistry/metabolism
MH  - Integrases/*metabolism
MH  - Models, Biological
MH  - Mutagenesis, Site-Directed/*methods
MH  - Organisms, Genetically Modified
MH  - Recombination, Genetic/physiology
MH  - Regulatory Sequences, Nucleic Acid/*genetics
MH  - Saccharomyces cerevisiae/*genetics/growth & development/metabolism
PMC - PMC3176630
MID - NIHMS250374
EDAT- 2009/10/06 06:00
MHDA- 2009/11/10 06:00
PMCR- 2011/09/20
CRDT- 2009/10/06 06:00
PHST- 2009/10/06 06:00 [entrez]
PHST- 2009/10/06 06:00 [pubmed]
PHST- 2009/11/10 06:00 [medline]
PHST- 2011/09/20 00:00 [pmc-release]
AID - 10.1007/978-1-59745-527-5_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2009;557:55-63. doi: 10.1007/978-1-59745-527-5_5.