PMID- 1980113
OWN - NLM
STAT- MEDLINE
DCOM- 19910220
LR  - 20171116
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 20
IP  - 12
DP  - 1990 Dec
TI  - A three-dimensional model system to study the interactions between human 
      leukocytes and endothelial cells.
PG  - 2775-81
AB  - Leukocyte adhesion to endothelial cells and migration into the subendothelial 
      matrix was studied with a three-dimensional model system, consisting of human 
      endothelial cells cultured on a loose collagen matrix. We developed a new method 
      to separate the endothelial cell monolayer and adhering leukocytes, from the 
      subendothelial matrix, allowing simultaneous analysis of leukocyte adhesion and 
      transendothelial migration. Monocytes adhered more avidly to untreated 
      endothelial cells than did neutrophils (2.5 +/- 0.3 vs. 1.0 +/- 0.2 leukocytes 
      per endothelial cell). Only a small fraction (10%-20%) of these leukocytes 
      migrated into the subendothelium. Pretreatment of endothelial cells with 
      interleukin 1 (IL 1) enhanced adhesion (20%), but not migration of monocytes. In 
      contrast, neutrophil adhesion was markedly and in a time-dependent manner 
      increased by IL 1 treatment (i.e. 200% after 6 h and 110% after 24 h of IL 1 
      treatment). Moreover, IL 1 pretreatment enhanced neutrophil migration twofold. 
      Activation of leukocytes with formyl-methionyl-leucyl-phenylalanine (fMLP) 
      enhanced both monocyte and neutrophil adhesion, but did not affect leukocyte 
      migration. Under all conditions, monocyte adhesion was only partly (30%-40%) 
      inhibited by monoclonal antibodies (mAb) against the common beta subunit of the 
      leukocyte-cell adhesion molecules (LeuCAM: CD18) and 25%-30% by mAb against the 
      alpha subunit of LFA-1 (CD11a). In contrast, mAb against the alpha subunits of 
      Mac-1 (CD11b) and p150.95 (CD11c) were hardly effective. fMLP-mediated neutrophil 
      adhesion was reduced to below baseline levels by anti-LeuCAM (CD18) mAb, whereas 
      the LeuCAM contribution in IL 1-mediated neutrophil adhesion was less pronounced 
      and varied in time. IL 1-mediated neutrophil migration, however, was completely 
      blocked by anti-LeuCAM mAb. fMLP-mediated neutrophil adhesion was inhibited by 
      mAb against the alpha subunits of Mac, while mAb against the alpha subunits of 
      LFA-1 and Mac-1 both reduced IL 1-mediated adherence. In summary, we describe a 
      novel leukocyte adhesion/migration method and demonstrate that the contribution 
      of the LeuCAM complex in leukocyte-endothelium interaction varies depending on 
      cell type and stimulus used.
FAU - Hakkert, B C
AU  - Hakkert BC
AD  - Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, 
      Amsterdam.
FAU - Rentenaar, J M
AU  - Rentenaar JM
FAU - Van Aken, W G
AU  - Van Aken WG
FAU - Roos, D
AU  - Roos D
FAU - Van Mourik, J A
AU  - Van Mourik JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (CD11 Antigens)
RN  - 0 (CD18 Antigens)
RN  - 0 (Interleukin-1)
RN  - 0 (Receptors, Leukocyte-Adhesion)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, CD/physiology
MH  - Antigens, Differentiation/physiology
MH  - CD11 Antigens
MH  - CD18 Antigens
MH  - *Cell Adhesion
MH  - *Cell Movement
MH  - Cells, Cultured
MH  - Collagen
MH  - Endothelium, Vascular/*cytology
MH  - Extracellular Matrix/physiology
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Leukocytes/*cytology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Receptors, Leukocyte-Adhesion/physiology
MH  - Time Factors
EDAT- 1990/12/01 00:00
MHDA- 1990/12/01 00:01
CRDT- 1990/12/01 00:00
PHST- 1990/12/01 00:00 [pubmed]
PHST- 1990/12/01 00:01 [medline]
PHST- 1990/12/01 00:00 [entrez]
AID - 10.1002/eji.1830201236 [doi]
PST - ppublish
SO  - Eur J Immunol. 1990 Dec;20(12):2775-81. doi: 10.1002/eji.1830201236.