PMID- 19803132
OWN - NLM
STAT- MEDLINE
DCOM- 20101005
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 3
DP  - 2009 May
TI  - [Determination of hormone multi-residues in animal tissues by gas 
      chromatography-mass spectrometry].
PG  - 294-8
AB  - A method of gas chromatography-mass spectrometry (GC-MS) for the simultaneous 
      determination of nine sex hormone residues, such as hexestrol, 
      diethylstilbestrol, dienestrol, etiocholan-3alpha-ol-17-one, epitestosterone, 
      estrone, estradiol, ethinylestradiol and estriol, in animal tissues was 
      developed. The sex hormones were extracted with acetonitrile, then cleaned-up 
      with a C18 solid-phase extraction (SPE) column. The microwave-assisted 
      derivatization of the target components with N,O-bis( trimethylsilyl) 
      trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS) (99:1, v/v) using 
      pyridine as solvent was performed, and then the derivatives were analyzed by 
      GC-MS. The limits of detection were 0.1-1 microg/kg for all hormones, and the 
      limits of quantification were 0.2-2 microg/kg. The average recoveries of sex 
      hormones were 68.8%-93.1%. The relative standard deviations (RSDs) of inter- and 
      intra-assay were 4.1%-22.3% and 3.1%-17.9%, respectively. The real sample tests 
      showed this method can be used for the sensitive and accurate determination of 
      multi-sex hormones residues in biological samples.
FAU - Lin, Weixuan
AU  - Lin W
AD  - Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China. 
      dlciqlwx@sina.com.cn
FAU - Dong, Weifeng
AU  - Dong W
FAU - Chen, Xi
AU  - Chen X
FAU - Tian, Miao
AU  - Tian M
FAU - Yu, Ling
AU  - Yu L
FAU - Zhao, Jinghong
AU  - Zhao J
FAU - Yang, Chunguang
AU  - Yang C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Gonadal Steroid Hormones)
RN  - 10BI795R7D (Hexestrol)
RN  - 481-30-1 (Epitestosterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Drug Residues/*analysis
MH  - Epitestosterone/analysis
MH  - Estradiol/analysis
MH  - Food Contamination/analysis
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Gonadal Steroid Hormones/*analysis
MH  - Hexestrol/analysis
MH  - Meat Products/*analysis
MH  - Swine
EDAT- 2009/10/07 06:00
MHDA- 2010/10/06 06:00
CRDT- 2009/10/07 06:00
PHST- 2009/10/07 06:00 [entrez]
PHST- 2009/10/07 06:00 [pubmed]
PHST- 2010/10/06 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 May;27(3):294-8.