PMID- 19803150
OWN - NLM
STAT- MEDLINE
DCOM- 20101005
LR  - 20161125
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 3
DP  - 2009 May
TI  - [Determination of optical purity of alpha-phenylethylamine by high performance 
      liquid chromatography with pre-column derivatization].
PG  - 376-8
AB  - A simple pre-column derivatization-high performance liquid chromatographic (HPLC) 
      method was established for the determination of optical purity of 
      alpha-phenylethylamine. The enantiomers of alpha-phenylethylamine were 
      derivatized with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate 
      (GITC). The resulted diastereoisomers were separated on an Agilent Zorbax C18 
      column (250 mm x 4.6 mm, 5 microm) with a mobile phase of methanol-phosphate 
      buffer (1.36 g/L aqueous solution of potassium dihydrogen phosphate, adjusted to 
      pH 3.0 with concentrated phosphoric acid) (58:42, v/v). The flow rate was set at 
      1.0 mL/min and the detection wavelength was set at 241 nm. The method was linear 
      from 0.15 - 15.0 mg/L for both enantiomers. The limit of detection and the limit 
      of quantification were 0.05 mg/L and 0.15 mg/L, respectively. The relative 
      standard deviations (RSDs) of inter- and intra-day determination were below 0.5%. 
      The method is easy to handle, accurate, and suitable for the quality control of 
      the optical purity of alpha-phenylethylamine.
FAU - Wang, Jinzhao
AU  - Wang J
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Zeng, Su
AU  - Zeng S
FAU - Wang, Danhua
AU  - Wang D
FAU - Hu, Gongyun
AU  - Hu G
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Phenethylamines)
RN  - HZ9DM6B2MT (1-phenethylamine)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Phenethylamines/*analysis/*chemistry
MH  - Photochemistry
MH  - Stereoisomerism
EDAT- 2009/10/07 06:00
MHDA- 2010/10/06 06:00
CRDT- 2009/10/07 06:00
PHST- 2009/10/07 06:00 [entrez]
PHST- 2009/10/07 06:00 [pubmed]
PHST- 2010/10/06 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 May;27(3):376-8.