PMID- 19804500
OWN - NLM
STAT- MEDLINE
DCOM- 20101101
LR  - 20211020
IS  - 1365-2893 (Electronic)
IS  - 1352-0504 (Print)
IS  - 1352-0504 (Linking)
VI  - 17
IP  - 8
DP  - 2010 Aug
TI  - Hepatitis C virus NS4 protein impairs the Th1 polarization of immature dendritic 
      cells.
PG  - 555-62
LID - 10.1111/j.1365-2893.2009.01213.x [doi]
AB  - Dendritic cells (DCs) in chronic hepatitis C patients display impaired function, 
      although the details remain unclear. To investigate the hepatitis C virus (HCV) 
      protein that has the most impact on DC function, we compared five recombinant 
      proteins and seven HCV protein genes in modulating DC phenotype and function. 
      Immature DCs (iDCs) were established from healthy donor peripheral blood 
      monocytes with granulocyte-macrophage colony stimulating factor (GM-CSF) and 
      IL-4. Lipopolysaccharide was used to establish mature DCs (mDCs). Cells were then 
      pulsed with HCV recombinant proteins or transfected with HCV plasmids and 
      subsequently assayed for cell surface marker expression by flow cytometry. For 
      cytokine and proliferative T-cell response analysis, DCs were cultured with 
      autologous CD4 T cells and tuberculin purified protein derivative (PPD). Mean 
      fluorescent intensity of CD86 was reduced in HCV protein-pulsed iDCs. 
      Proliferative T-cell responses and Th1 cytokine concentrations were reduced with 
      HCV nonstructural proteins (NS), particularly with HCV NS4. HCV nonstructural 
      proteins, particularly NS4, change the iDC phenotype and reduce antigen-specific 
      T-cell stimulatory function with Th1 cytokine reductions.
FAU - Takaki, A
AU  - Takaki A
AD  - Department of Gastroenterology and Hepatology, Okayama University Graduate School 
      of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan. 
      akitaka@md.okayama-u.ac.jp
FAU - Tatsukawa, M
AU  - Tatsukawa M
FAU - Iwasaki, Y
AU  - Iwasaki Y
FAU - Koike, K
AU  - Koike K
FAU - Noguchi, Y
AU  - Noguchi Y
FAU - Shiraha, H
AU  - Shiraha H
FAU - Sakaguchi, K
AU  - Sakaguchi K
FAU - Nakayama, E
AU  - Nakayama E
FAU - Yamamoto, K
AU  - Yamamoto K
LA  - eng
PT  - Journal Article
DEP - 20091004
PL  - England
TA  - J Viral Hepat
JT  - Journal of viral hepatitis
JID - 9435672
RN  - 0 (Cytokines)
RN  - 0 (NS4 protein, hepatitis C virus)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Adult
MH  - Cell Polarity/immunology
MH  - Cytokines/immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Flow Cytometry
MH  - Hepacivirus/*immunology
MH  - Hepatitis C, Chronic/*immunology/virology
MH  - Humans
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Plasmids/immunology
MH  - Recombinant Proteins/*immunology
MH  - Statistics, Nonparametric
MH  - Th1 Cells/*immunology
MH  - Viral Nonstructural Proteins/*immunology
PMC - PMC2916225
EDAT- 2009/10/07 06:00
MHDA- 2010/11/03 06:00
CRDT- 2009/10/07 06:00
PHST- 2009/10/07 06:00 [entrez]
PHST- 2009/10/07 06:00 [pubmed]
PHST- 2010/11/03 06:00 [medline]
AID - JVH1213 [pii]
AID - 10.1111/j.1365-2893.2009.01213.x [doi]
PST - ppublish
SO  - J Viral Hepat. 2010 Aug;17(8):555-62. doi: 10.1111/j.1365-2893.2009.01213.x. Epub 
      2009 Oct 4.