PMID- 19806187
OWN - NLM
STAT- MEDLINE
DCOM- 20100312
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 4
IP  - 10
DP  - 2009 Oct 6
TI  - Dendritic cell cross-priming is essential for immune responses to Listeria 
      monocytogenes.
PG  - e7210
LID - 10.1371/journal.pone.0007210 [doi]
LID - e7210
AB  - Cross-presentation is now recognized as a major mechanism for initiating CD8 T 
      cell responses to virus and tumor antigens in vivo. It provides an elegant 
      mechanism that allows relatively few Dendritic cells (DCs) to initiate primary 
      immune responses while avoiding the consumptive nature of pathogenic infection. 
      CD8 T cells play a major role in anti-bacterial immune responses; however, the 
      contribution of cross-presentation for priming CD8 T cell responses to bacteria, 
      in vivo, is not well established. Listeria monocytogenes (Listeria) is the 
      causative agent of Listeriosis, an opportunistic food-borne bacterial infection 
      that poses a significant public health risk. Here, we employ a transgenic mouse 
      model in which cross-presentation is uniquely inactivated, to investigate 
      cross-priming during primary Listeria infection. We show that cross-priming 
      deficient mice are severely compromised in their ability to generate 
      antigen-specific T cells to stimulate MHC I-restricted CTL responses following 
      Listeria infection. The defect in generation of Listeria-elicited CD8 T cell 
      responses is also apparent in vitro. However, in this setting, the endogenous 
      route of processing Listeria-derived antigens is predominant. This reveals a new 
      experimental dichotomy whereby functional sampling of Listeria-derived antigens 
      in vivo but not in vitro is dependent on cross-presentation of exogenously 
      derived antigen. Thus, under normal physiological circumstances, 
      cross-presentation is demonstrated to play an essential role in priming CD8 T 
      cell responses to bacteria.
FAU - Reinicke, Anna T
AU  - Reinicke AT
AD  - The Biomedical Research Centre, Michael Smith Laboratories, Department of Medical 
      Genetics, Microbiology and Immunology, The University of British Columbia, 
      Vancouver, British Columbia, Canada.
FAU - Omilusik, Kyla D
AU  - Omilusik KD
FAU - Basha, Genc
AU  - Basha G
FAU - Jefferies, Wilfred A
AU  - Jefferies WA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091006
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Bone Marrow Cells/cytology
MH  - CD8-Positive T-Lymphocytes/immunology/microbiology
MH  - Cell Proliferation
MH  - Cross-Priming/immunology
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Immune System
MH  - Listeria monocytogenes/*immunology
MH  - Listeriosis/immunology/microbiology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
PMC - PMC2751817
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2009/10/07 06:00
MHDA- 2010/03/13 06:00
PMCR- 2009/10/06
CRDT- 2009/10/07 06:00
PHST- 2009/05/19 00:00 [received]
PHST- 2009/08/09 00:00 [accepted]
PHST- 2009/10/07 06:00 [entrez]
PHST- 2009/10/07 06:00 [pubmed]
PHST- 2010/03/13 06:00 [medline]
PHST- 2009/10/06 00:00 [pmc-release]
AID - 09-PONE-RA-10450R1 [pii]
AID - 10.1371/journal.pone.0007210 [doi]
PST - epublish
SO  - PLoS One. 2009 Oct 6;4(10):e7210. doi: 10.1371/journal.pone.0007210.