PMID- 19806405
OWN - NLM
STAT- MEDLINE
DCOM- 20100629
LR  - 20211020
IS  - 1860-2002 (Electronic)
IS  - 1536-1632 (Linking)
VI  - 12
IP  - 2
DP  - 2010 Apr
TI  - Detection of experimentally induced pulmonary granuloma inflammation in monocyte 
      chemoattractant protein-1 reporter mice.
PG  - 163-73
LID - 10.1007/s11307-009-0261-9 [doi]
AB  - PURPOSE: Among different chemokines, monocyte chemoattractant protein-1 (MCP-1) 
      plays an important role in inflammatory disorders of lung. In response to 
      stimuli, MCP-1 increases its transcription as an immediate early gene. In this 
      paper, we describe the MCP-1-enhanced green fluorescent protein(EGFP) transgenic 
      mouse in which EGFP expression is driven by human MCP-1 promoter and mimics the 
      MCP-1 expression in situ. Thus, the MCP-1 reporter mouse model is designed to 
      facilitate a better understanding of its role in various diseases. We employed 
      this mouse model in a pulmonary granulomatous inflammation model using 
      intratracheal instillation of Sephadex (SDX) beads and compared the EGFP reporter 
      expression to endogenous MCP-1 expression through the course of inflammation. 
      PROCEDURES: We analyzed the temporal pattern of SDX-induced infiltration of 
      inflammatory cells in lung and in bronchoalveolar lavage fluid (BALF). The 
      changes in tissue fluorescence, gene, and protein expressions for both MCP-1 and 
      EGFP were analyzed. RESULTS: SDX instillation caused massive infiltration of 
      inflammatory cells in BALF and lung tissue at the end of day 3. There was an 
      increase of fluorescence in SDX-treated lung and BALF cells. By using 
      lipopolysaccharide-induced systemic inflammation model, increase of fluorescence 
      was found in bone marrow Gr-1(+) cells with high Mac-1 expression. MCP-1 and EGFP 
      gene expression and MCP-1 protein level were increased after day 1, peaked at day 
      3, and declined toward basal levels at day 5. In contrast, EGFP protein level 
      peaked after day 3 and remained elevated after day 5. Immunohistochemical 
      staining revealed the MCP-1 and EGFP expression primarily at alveolar 
      macrophages, macrophages infiltrating the granulomatous lesions and in 
      bronchiolar epithelial cells. CONCLUSIONS: By using a pulmonary granuloma model, 
      we showed that EGFP transgene reporter expression in MCP-1-EGFP mouse was 
      correlated to the endogenous MCP-1 induction. The establishment of this mouse 
      model will provide a valuable tool for monitoring the activation of 
      monocytes/macrophages and facilitate the studies on the roles of MCP-1 gene in 
      various inflammatory diseases.
FAU - Rajasekaran, Subbiah
AU  - Rajasekaran S
AD  - Division of Medical Engineering Research, National Health Research Institutes, 
      Zhunan Town, Miaoli County, Taiwan, Republic of China.
FAU - Kao, Vivia Yu-Ying
AU  - Kao VY
FAU - Chen, Mei-Ru
AU  - Chen MR
FAU - Yang, Alex Liang-Tung
AU  - Yang AL
FAU - Hsu, Ching-Han
AU  - Hsu CH
FAU - Chen, Chin-Tu
AU  - Chen CT
FAU - Lin, Kurt Ming-Chao
AU  - Lin KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091006
PL  - United States
TA  - Mol Imaging Biol
JT  - Molecular imaging and biology
JID - 101125610
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Disease Models, Animal
MH  - Flow Cytometry
MH  - Genes, Reporter/*genetics
MH  - Genetic Vectors/genetics
MH  - Genotype
MH  - Granuloma, Respiratory Tract/chemically induced/*complications/*diagnosis
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Leukocyte Count
MH  - Lipopolysaccharides/pharmacology
MH  - Lung/drug effects/metabolism/pathology
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Imaging
MH  - Pneumonia/chemically induced/*complications/*diagnosis
EDAT- 2009/10/07 06:00
MHDA- 2010/06/30 06:00
CRDT- 2009/10/07 06:00
PHST- 2009/01/26 00:00 [received]
PHST- 2009/04/28 00:00 [accepted]
PHST- 2009/04/01 00:00 [revised]
PHST- 2009/10/07 06:00 [entrez]
PHST- 2009/10/07 06:00 [pubmed]
PHST- 2010/06/30 06:00 [medline]
AID - 10.1007/s11307-009-0261-9 [doi]
PST - ppublish
SO  - Mol Imaging Biol. 2010 Apr;12(2):163-73. doi: 10.1007/s11307-009-0261-9. Epub 
      2009 Oct 6.