PMID- 19808074
OWN - NLM
STAT- MEDLINE
DCOM- 20100121
LR  - 20191210
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 71
IP  - 1
DP  - 2010 Jan
TI  - Improved Luminex-based human leukocyte antigen-specific antibody screening using 
      dithiothreitol-treated sera.
PG  - 45-9
LID - 10.1016/j.humimm.2009.09.358 [doi]
AB  - Solid-phase binding assays using purified human leukocyte antigen (HLA) allow the 
      accurate identification and characterization of HLA-specific antibodies in organ 
      transplant patients, but sera may contain factors that block the detection of 
      clinically relevant alloantibodies. The effect of treating patient sera with 
      dithiothreitol (DTT) on alloantibody detection was examined. In 49 sera submitted 
      for routine HLA-specific antibody monitoring, DTT made little difference to 
      immunoglobulin G HLA-specific antibody detection using LABScreen HLA class I and 
      class II mixed beads. However, in sera submitted for antibody identification 
      using single antigen beads (SAB), DTT markedly increased (>twofold increased 
      median fluorescence intensity) antibody binding to HLA class I and/or class II 
      specificities in 14 of 76 (18%) patient sera. In a cohort of highly sensitized 
      patients, treatment of sera with DTT enhanced antibody-binding levels in 14 of 15 
      (93%) sera. This study highlights the need to consider routine testing of sera 
      with and without DTT for analysis of HLA-specific antibodies by SAB.
FAU - Kosmoliaptsis, Vasilis
AU  - Kosmoliaptsis V
AD  - Tissue Typing Laboratory, Cambridge University Hospitals NHS Foundation Trust, 
      Addenbrooke's Hospital, Hills Road, Cambridge CB2 0QQ, United Kingdom.
FAU - O'Rourke, Cheryl
AU  - O'Rourke C
FAU - Bradley, J Andrew
AU  - Bradley JA
FAU - Taylor, Craig J
AU  - Taylor CJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antibodies)
RN  - 0 (HLA Antigens)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Antibodies/*blood/*immunology
MH  - Dithiothreitol/*chemistry
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Immunoassay/*methods
MH  - Kidney Transplantation/immunology
MH  - Luminescent Measurements/*methods
EDAT- 2009/10/08 06:00
MHDA- 2010/01/22 06:00
CRDT- 2009/10/08 06:00
PHST- 2009/08/11 00:00 [received]
PHST- 2009/09/10 00:00 [revised]
PHST- 2009/09/30 00:00 [accepted]
PHST- 2009/10/08 06:00 [entrez]
PHST- 2009/10/08 06:00 [pubmed]
PHST- 2010/01/22 06:00 [medline]
AID - S0198-8859(09)00593-X [pii]
AID - 10.1016/j.humimm.2009.09.358 [doi]
PST - ppublish
SO  - Hum Immunol. 2010 Jan;71(1):45-9. doi: 10.1016/j.humimm.2009.09.358.