PMID- 19811790
OWN - NLM
STAT- MEDLINE
DCOM- 20100323
LR  - 20220310
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 159
IP  - 1
DP  - 2010 Mar
TI  - Hydrogen sulfide protects against ischemia-reperfusion injury in an in vitro 
      model of cutaneous tissue transplantation.
PG  - 451-5
LID - 10.1016/j.jss.2009.05.010 [doi]
AB  - BACKGROUND: Ischemia-reperfusion injury (IRI) is a source of morbidity and 
      mortality in many clinical scenarios, and has as one of its many consequences the 
      induction of cellular apoptosis. Hydrogen sulfide (H2S) may decrease cellular 
      metabolism in a reversible, nontoxic manner. An in vitro model of cutaneous 
      tissue transplantation was developed to assess whether H2S could ameliorate 
      cellular injury caused by IRI. METHODS: Human umbilical vein endothelial cells 
      (HUVECs) were treated with media containing NaHS (0, 10 microM, 100 microM, or 1 
      mM) and exposed to normoxia (21% oxygen), hypoxia (1%), or anoxia (0%). Cells 
      were then returned to normoxia, and apoptosis was quantified using a terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. 
      Fibroblasts (3T3s) were treated with H2S and exposed to anoxia in a similar 
      fashion. RESULTS: Treatment with H2S resulted in a significant decrease in 
      apoptosis in HUVECs and 3T3s subjected to IRI. Toxicity of H2S was not observed, 
      although the protective effect was less evident at higher doses. CONCLUSION: This 
      is the first study to examine H2S and the cellular components of cutaneous flaps 
      in the setting of IRI. Our results demonstrate that H2S significantly decreases 
      apoptosis in vitro in the setting of IRI. These data suggest H2S may mitigate IRI 
      in vivo, and, therefore, has potential as a therapy for improving tissue 
      survivability in clinical scenarios.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Henderson, Peter W
AU  - Henderson PW
AD  - Weill Cornell Medical College, New York, New York 10065, USA.
FAU - Singh, Sunil P
AU  - Singh SP
FAU - Belkin, Daniel
AU  - Belkin D
FAU - Nagineni, Vamsi
AU  - Nagineni V
FAU - Weinstein, Andrew L
AU  - Weinstein AL
FAU - Weissich, Jacob
AU  - Weissich J
FAU - Spector, Jason A
AU  - Spector JA
LA  - eng
PT  - Journal Article
DEP - 20090606
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - *Cytoprotection
MH  - Endothelial Cells/drug effects
MH  - Fibroblasts/drug effects
MH  - Humans
MH  - Hydrogen Sulfide/pharmacology/*therapeutic use
MH  - Mice
MH  - Reperfusion Injury/*prevention & control
MH  - *Skin Transplantation
MH  - Surgical Flaps
EDAT- 2009/10/09 06:00
MHDA- 2010/03/24 06:00
CRDT- 2009/10/09 06:00
PHST- 2009/01/09 00:00 [received]
PHST- 2009/04/22 00:00 [revised]
PHST- 2009/05/01 00:00 [accepted]
PHST- 2009/10/09 06:00 [entrez]
PHST- 2009/10/09 06:00 [pubmed]
PHST- 2010/03/24 06:00 [medline]
AID - S0022-4804(09)00265-0 [pii]
AID - 10.1016/j.jss.2009.05.010 [doi]
PST - ppublish
SO  - J Surg Res. 2010 Mar;159(1):451-5. doi: 10.1016/j.jss.2009.05.010. Epub 2009 Jun 
      6.