PMID- 19816239
OWN - NLM
STAT- MEDLINE
DCOM- 20100325
LR  - 20211020
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 67
IP  - 1
DP  - 2010 Jan
TI  - Airway injury from initiating ventilation in preterm sheep.
PG  - 60-5
LID - 10.1203/PDR.0b013e3181c1b09e [doi]
AB  - Premature infants exposed to ventilation are at risk of developing 
      bronchopulmonary dysplasia and persistent lung disease in childhood. We report 
      where injury occurred within the lung after brief ventilation at birth. Preterm 
      sheep (129 d gestation) were ventilated with an escalating tidal volume to 15 
      mL/kg by 15 min to injure the lungs, with the placental circulation intact 
      (fetal) or after delivery (newborn). Fetal lambs were returned to the uterus for 
      2 h 45 min, whereas newborn lambs were maintained with gentle ventilatory support 
      for the same period. The control group was not ventilated. Bronchoalveolar lavage 
      fluid (BALF) and lung tissue were analyzed. In both fetal and newborn lambs, 
      ventilation caused bronchial epithelial disruption in medium-sized airways. Early 
      growth response protein 1 (Egr-1), monocyte chemotactic protein 1 (MCP-1), IL-6, 
      and IL-1beta mRNA increased in the lung tissue from fetal and newborn lambs. 
      Egr-1, MCP-1, and IL-6 mRNA were induced in mesenchymal cells surrounding small 
      airways, whereas IL-1beta mRNA localized to the epithelium of medium/small 
      airways. Ventilation caused loss of heat shock protein 70 (HSP70) mRNA from the 
      bronchial epithelium, but induced mRNA in the smooth muscle surrounding large 
      airways. HSP70 protein decreased in the lung tissue and increased in BALF with 
      ventilation. Initiation of ventilation induced a stress response and inflammatory 
      cytokines in small and medium-sized airways.
FAU - Hillman, Noah H
AU  - Hillman NH
AD  - Division of Pulmonary Biology, University of Cincinnati, Cincinnati, Ohio 45229, 
      USA. Noah.Hillman@cchmc.org
FAU - Kallapur, Suhas G
AU  - Kallapur SG
FAU - Pillow, J Jane
AU  - Pillow JJ
FAU - Moss, Timothy J M
AU  - Moss TJ
FAU - Polglase, Graeme R
AU  - Polglase GR
FAU - Nitsos, Ilias
AU  - Nitsos I
FAU - Jobe, Alan H
AU  - Jobe AH
LA  - eng
GR  - HD07541/HD/NICHD NIH HHS/United States
GR  - K08 HL097085-01/HL/NHLBI NIH HHS/United States
GR  - T32 HD007541/HD/NICHD NIH HHS/United States
GR  - K08 HL097085/HL/NHLBI NIH HHS/United States
GR  - R01 HD012714/HD/NICHD NIH HHS/United States
GR  - HD-12714/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bronchoalveolar Lavage Fluid
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Inflammation Mediators/metabolism
MH  - Premature Birth
MH  - *Respiration, Artificial
MH  - Sheep
MH  - Trachea/*injuries
PMC - PMC2795027
MID - NIHMS161272
EDAT- 2009/10/10 06:00
MHDA- 2010/03/26 06:00
PMCR- 2011/01/01
CRDT- 2009/10/10 06:00
PHST- 2009/10/10 06:00 [entrez]
PHST- 2009/10/10 06:00 [pubmed]
PHST- 2010/03/26 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - 10.1203/PDR.0b013e3181c1b09e [doi]
PST - ppublish
SO  - Pediatr Res. 2010 Jan;67(1):60-5. doi: 10.1203/PDR.0b013e3181c1b09e.