PMID- 19816414
OWN - NLM
STAT- MEDLINE
DCOM- 20100902
LR  - 20240404
IS  - 1930-739X (Electronic)
IS  - 1930-7381 (Print)
IS  - 1930-7381 (Linking)
VI  - 18
IP  - 4
DP  - 2010 Apr
TI  - Retinol-binding protein 4 (RBP4) protein expression is increased in omental 
      adipose tissue of severely obese patients.
PG  - 663-6
LID - 10.1038/oby.2009.328 [doi]
AB  - Visceral fat has been linked to insulin resistance and type 2 diabetes mellitus 
      (T2DM); and emerging data links RBP4 gene expression in adipose tissue with 
      insulin resistance. In this study, we examined RBP4 protein expression in omental 
      adipose tissue obtained from 24 severely obese patients undergoing bariatric 
      surgery, and 10 lean controls (4 males/6 females, BMI = 23.2 +/- 1.5 kg/m(2)) 
      undergoing elective abdominal surgeries. Twelve of the obese patients had T2DM (2 
      males/10 females, BMI: 44.7 +/- 1.5 kg/m(2)) and 12 had normal glucose tolerance 
      (NGT: 4 males/8 females, BMI: 47.6 +/- 1.9 kg/m(2)). Adipose RBP4, glucose 
      transport protein-4 (GLUT4), and p85 protein expression were determined by 
      western blot. Blood samples from the bariatric patients were analyzed for serum 
      RBP4, total cholesterol, triglycerides, and glucose. Adipose RBP4 protein 
      expression (NGT: 11.0 +/- 0.6; T2DM: 11.8 +/- 0.7; lean: 8.7 +/- 0.8 arbitrary 
      units) was significantly increased in both NGT (P = 0.03) and T2DM (P = 0.005), 
      compared to lean controls. GLUT4 protein was decreased in both NGT (P = 0.02) and 
      T2DM (P = 0.03), and p85 expression was increased in T2DM subjects, compared to 
      NGT (P = 0.03) and lean controls (P = 0.003). Regression analysis showed a strong 
      correlation between adipose RBP4 protein and BMI for all subjects, as well as 
      between adipose RBP4 and fasting glucose levels in T2DM subjects (r = 0.76, P = 
      0.004). Further, in T2DM, serum RBP4 was correlated with p85 expression (r = 
      0.68, P = 0.01), and adipose RBP4 protein trended toward an association with p85 
      protein (r = 0.55, P = 0.06). These data suggest that RBP4 may regulate 
      adiposity, and p85 expression in obese-T2DM, thus providing a link to impaired 
      insulin signaling and diabetes in severely obese patients.
FAU - Kelly, Karen R
AU  - Kelly KR
AD  - Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, 
      Cleveland, Ohio, USA.
FAU - Kashyap, Sangeeta R
AU  - Kashyap SR
FAU - O'Leary, Valerie B
AU  - O'Leary VB
FAU - Major, Jennifer
AU  - Major J
FAU - Schauer, Philip R
AU  - Schauer PR
FAU - Kirwan, John P
AU  - Kirwan JP
LA  - eng
GR  - UL1 RR024989/RR/NCRR NIH HHS/United States
GR  - M01 RR018390-056219/RR/NCRR NIH HHS/United States
GR  - T32DK007319/DK/NIDDK NIH HHS/United States
GR  - RR018390/RR/NCRR NIH HHS/United States
GR  - T32 DK007319/DK/NIDDK NIH HHS/United States
GR  - AG12834/AG/NIA NIH HHS/United States
GR  - K12 RR023264/RR/NCRR NIH HHS/United States
GR  - K12RR023264/RR/NCRR NIH HHS/United States
GR  - M01 RR018390/RR/NCRR NIH HHS/United States
GR  - UL1RR024989/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20091008
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Blood Glucose)
RN  - 0 (Blood Proteins)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (RBP4 protein, human)
RN  - 0 (Retinol-Binding Proteins, Plasma)
SB  - IM
MH  - Adiposity/*physiology
MH  - Blood Glucose/*metabolism
MH  - Blood Proteins/metabolism
MH  - *Body Mass Index
MH  - Diabetes Mellitus, Type 2/complications/*metabolism
MH  - Female
MH  - Gastric Bypass
MH  - Glucose Transporter Type 4/metabolism
MH  - Humans
MH  - Insulin
MH  - Intra-Abdominal Fat/*metabolism
MH  - Male
MH  - Obesity/complications/*metabolism/surgery
MH  - Omentum
MH  - Retinol-Binding Proteins, Plasma/*metabolism
MH  - Signal Transduction
PMC - PMC2919818
MID - NIHMS225707
EDAT- 2009/10/10 06:00
MHDA- 2010/09/03 06:00
PMCR- 2010/08/11
CRDT- 2009/10/10 06:00
PHST- 2009/10/10 06:00 [entrez]
PHST- 2009/10/10 06:00 [pubmed]
PHST- 2010/09/03 06:00 [medline]
PHST- 2010/08/11 00:00 [pmc-release]
AID - oby2009328 [pii]
AID - 10.1038/oby.2009.328 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2010 Apr;18(4):663-6. doi: 10.1038/oby.2009.328. Epub 
      2009 Oct 8.