PMID- 19816782
OWN - NLM
STAT- MEDLINE
DCOM- 20101202
LR  - 20211020
IS  - 1573-9368 (Electronic)
IS  - 0962-8819 (Linking)
VI  - 19
IP  - 4
DP  - 2010 Aug
TI  - The 5' untranslated region of the VR-ACS1 mRNA acts as a strong translational 
      enhancer in plants.
PG  - 667-74
LID - 10.1007/s11248-009-9332-6 [doi]
AB  - The structure and function of untranslated mRNA leader sequences and their role 
      in controlling gene expression remains poorly understood. Previous research has 
      suggested that the 5' untranslated region (5'UTR) of the Vigna radiata 
      aminocyclopropane-1-carboxylate synthase synthase (VR-ACS1) gene may function as 
      a translational enhancer in plants. To test such hypothesis we compared the 
      translation enhancing properties of three different 5'UTRs; those from the 
      VR-ACS1, the chlorophyll a/b binding gene from petunia (Cab22L; a known 
      translational enhancer) and the Vigna radiata pectinacetylesterase gene (PAE; 
      used as control). Identical constructs in which the coding region of the 
      beta-glucuronidase (GUS) gene was fused to each of the three 5'UTRs and placed 
      under the control of the cauliflower mosaic virus 35S promoter were prepared. 
      Transient expression assays in tobacco cell cultures and mung bean leaves showed 
      that the VR-ACS1 and Cab22L 5'UTRs directed higher levels of GUS activity than 
      the PAE 5'UTR. Analysis of transgenic Arabidopsis thaliana seedlings, as well as 
      different tissues from mature plants, confirmed that while transcript levels were 
      equivalent for all constructs, the 5'UTRs from the VR-ACS1 and Cab22L genes can 
      increase GUS activity twofold to fivefold compared to the PAE 5'UTR, therefore 
      confirming the translational enhancing properties of the VR-ACS1 5'UTR.
FAU - Wever, Willem
AU  - Wever W
AD  - Plant Genetic Engineering Laboratory, School of Biological Sciences, University 
      of Queensland, Brisbane, Queensland, 4072, Australia.
FAU - McCallum, Emily J
AU  - McCallum EJ
FAU - Chakravorty, David
AU  - Chakravorty D
FAU - Cazzonelli, Christopher I
AU  - Cazzonelli CI
FAU - Botella, Jose R
AU  - Botella JR
LA  - eng
PT  - Journal Article
DEP - 20091009
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Cyclopropanes)
RN  - 0 (RNA, Messenger)
RN  - 008803351S (cyclopropanecarboxylic acid)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 3.1.- (Esterases)
RN  - EC 3.1.1.- (pectinacetylesterase)
RN  - EC 3.2.1.31 (Glucuronidase)
RN  - EC 4.- (Lyases)
SB  - IM
MH  - *5' Untranslated Regions/physiology
MH  - Cyclopropanes/metabolism
MH  - Enhancer Elements, Genetic/*genetics
MH  - Esterases/genetics/metabolism
MH  - Fabaceae/*genetics/metabolism
MH  - *Gene Expression Regulation, Plant
MH  - Genes, Plant/genetics
MH  - Glucuronidase/genetics/metabolism
MH  - Lyases/genetics/metabolism
MH  - Methyltransferases/*genetics
MH  - Plants/genetics/metabolism
MH  - Plants, Genetically Modified
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2009/10/10 06:00
MHDA- 2010/12/14 06:00
CRDT- 2009/10/10 06:00
PHST- 2009/07/01 00:00 [received]
PHST- 2009/09/25 00:00 [accepted]
PHST- 2009/10/10 06:00 [entrez]
PHST- 2009/10/10 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1007/s11248-009-9332-6 [doi]
PST - ppublish
SO  - Transgenic Res. 2010 Aug;19(4):667-74. doi: 10.1007/s11248-009-9332-6. Epub 2009 
      Oct 9.