PMID- 19818076
OWN - NLM
STAT- MEDLINE
DCOM- 20100701
LR  - 20191210
IS  - 1464-410X (Electronic)
IS  - 1464-4096 (Linking)
VI  - 105
IP  - 10
DP  - 2010 May
TI  - Urinary fluorescence in situ hybridization assay for detecting urothelial 
      carcinoma in Taiwanese patients.
PG  - 1413-6
LID - 10.1111/j.1464-410X.2009.08917.x [doi]
AB  - OBJECTIVES: To investigate the cytogenetic marker detected by fluorescence in 
      situ hybridization (FISH; UroVysion, Vysis, Inc., Abbott Laboratories, Des 
      Plaines, IL, USA) in the diagnosis of bladder cancer and upper tract urothelial 
      carcinoma (UC) in Taiwanese patients, as FISH has been used in Western countries 
      for detecting UC, but there are limited results in Asian populations. PATIENTS 
      AND METHODS: We analysed polyploidy of chromosome 3, 7, 17 and aneuploidy of 
      chromosome 21, using uroepithelial cells collected at the first void or by 
      instrumental extraction of urine, for bladder cancer, and shedding cells from the 
      upper tract flushed by normal saline via ureteric catheterization or 
      ureterorenoscopy. The criteria for positive tumour cells included three or more 
      positive staining in two or more chromosomes showing polyploidy or <50% staining 
      of the chromosome 9p21. RESULTS: In all, 32 patients with bladder UC and 12 with 
      upper tract UC were assessed. The overall sensitivity for bladder cancer by 
      UroVysion was 96.8%. The sensitivity of the cytology test was 36% for UC of the 
      bladder. The sensitivity for UroVysion in upper tract UC was 12/12 but the 
      specificity was three of nine. CONCLUSIONS: This preliminary report shows that 
      UroVysion was a sensitive screening method for UC of the bladder and upper 
      urinary tract.
FAU - Chuang, Kun-Lung
AU  - Chuang KL
AD  - Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Chang 
      Gung University, Taoyuan, Taiwan.
FAU - Chuang, Hun-Chang
AU  - Chuang HC
FAU - Ng, Kwai-Fong
AU  - Ng KF
FAU - Chang, Ying-Hsun
AU  - Chang YH
FAU - Wu, Chun-Te
AU  - Wu CT
FAU - Chuang, Cheng-Keng
AU  - Chuang CK
FAU - Liao, Shuen-Kuei
AU  - Liao SK
FAU - Pang, See-Tong
AU  - Pang ST
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091010
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Aneuploidy
MH  - Carcinoma, Transitional Cell/*diagnosis/genetics
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Polyploidy
MH  - Sensitivity and Specificity
MH  - Taiwan
MH  - Urinary Bladder Neoplasms/*diagnosis/genetics
MH  - Urothelium
EDAT- 2009/10/13 06:00
MHDA- 2010/07/02 06:00
CRDT- 2009/10/13 06:00
PHST- 2009/10/13 06:00 [entrez]
PHST- 2009/10/13 06:00 [pubmed]
PHST- 2010/07/02 06:00 [medline]
AID - BJU8917 [pii]
AID - 10.1111/j.1464-410X.2009.08917.x [doi]
PST - ppublish
SO  - BJU Int. 2010 May;105(10):1413-6. doi: 10.1111/j.1464-410X.2009.08917.x. Epub 
      2009 Oct 10.