PMID- 19818766
OWN - NLM
STAT- MEDLINE
DCOM- 20100225
LR  - 20171116
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 88
IP  - 1
DP  - 2010 Feb
TI  - Establishment of shared antigen reactive cytotoxic T lymphocyte using 
      co-stimulatory molecule introduced autologous cancer cells.
PG  - 128-32
LID - 10.1016/j.yexmp.2009.09.021 [doi]
AB  - Cytotoxic T lymphocytes (CTLs) play an essential role in immunological responses 
      for tumor rejection. In the past decade, many tumor-associated antigens (TAAs) 
      have been identified predominantly in melanomas. Several clinical trials based on 
      such antigenic peptides with or without adjuvants brought about partially 
      favorable results, suggesting that identification of more immunogenic TAAs is 
      needed. We show here the successful establishment of human leukocyte antigen 
      (HLA)-A24-restricted CTL (TcLHK2 line1) from a pleural effusion of lung cancer 
      patient, using B7.1 (CD80) transduced autologous lung cancer cells as an 
      antigen-presenting cell (APC). TcLHK2 line1 recognized autologous lung 
      adenocarcinoma cell line LHK2 in an HLA-A24-restricted fashion. Moreover, this 
      CTL line also recognized allogeneic HLA-A24-positive lung adenocarcinoma cell 
      line, gastric carcinoma cell line and melanoma cell line. These data raise the 
      possibility that co-stimulatory molecule B7.1 (CD80) plays important role to 
      overcome the immunological tolerance. Furthermore, TcLHK2 line1 is a useful tool 
      for the identification of widely expressed shared antigens restricted by HLA-A24. 
      Further analysis of this CTL and autologous cancer cell line will bring about 
      novel TAAs.
CI  - Copyright 2009 Elsevier Inc. All rights reserved.
FAU - Hirohashi, Yoshihiko
AU  - Hirohashi Y
AD  - Department of Pathology, Sapporo Medical University School of Medicine, South-1 
      West-17, chuo-ku, Sapporo 060-8556, Japan. hirohash@sapmed.ac.jp
FAU - Torigoe, Toshihiko
AU  - Torigoe T
FAU - Hirai, Itaru
AU  - Hirai I
FAU - Tamura, Yasuaki
AU  - Tamura Y
FAU - Nakatsugawa, Munehide
AU  - Nakatsugawa M
FAU - Inoue, Yuuji
AU  - Inoue Y
FAU - Kanaseki, Takayuki
AU  - Kanaseki T
FAU - Kamiguchi, Kenjiro
AU  - Kamiguchi K
FAU - Ikeda, Hideyuki
AU  - Ikeda H
FAU - Sasaki, Aya
AU  - Sasaki A
FAU - Yamanaka, Noboru
AU  - Yamanaka N
FAU - Sato, Noriyuki
AU  - Sato N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091008
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (B7-1 Antigen)
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
SB  - IM
MH  - Adenocarcinoma/immunology/*pathology
MH  - Aged
MH  - Antigen-Presenting Cells
MH  - Antigens, Neoplasm/immunology
MH  - B7-1 Antigen/immunology
MH  - Cell Line, Tumor
MH  - Cytotoxicity, Immunologic/immunology
MH  - Epitopes/immunology
MH  - HLA-A Antigens/immunology
MH  - HLA-A24 Antigen
MH  - Humans
MH  - Immune Tolerance
MH  - Lung Neoplasms/immunology/*pathology
MH  - Male
MH  - Melanoma/immunology/pathology
MH  - Stomach Neoplasms/immunology/pathology
MH  - T-Lymphocytes, Cytotoxic/*cytology/immunology
EDAT- 2009/10/13 06:00
MHDA- 2010/02/26 06:00
CRDT- 2009/10/13 06:00
PHST- 2009/03/03 00:00 [received]
PHST- 2009/09/24 00:00 [revised]
PHST- 2009/09/26 00:00 [accepted]
PHST- 2009/10/13 06:00 [entrez]
PHST- 2009/10/13 06:00 [pubmed]
PHST- 2010/02/26 06:00 [medline]
AID - S0014-4800(09)00134-8 [pii]
AID - 10.1016/j.yexmp.2009.09.021 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2010 Feb;88(1):128-32. doi: 10.1016/j.yexmp.2009.09.021. Epub 
      2009 Oct 8.