PMID- 19819989
OWN - NLM
STAT- MEDLINE
DCOM- 20100217
LR  - 20240109
IS  - 1944-9917 (Electronic)
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 23
IP  - 11
DP  - 2009 Nov
TI  - MicroRNA 132 regulates nutritional stress-induced chemokine production through 
      repression of SirT1.
PG  - 1876-84
LID - 10.1210/me.2009-0117 [doi]
AB  - Human adipose tissue secretes a number of proinflammatory mediators that may 
      contribute to the pathophysiology of obesity-related disorders. Understanding the 
      regulatory pathways that control their production is paramount to developing 
      effective therapeutics to treat these diseases. Using primary human 
      adipose-derived stem cells as a source of preadipocytes and in vitro 
      differentiated adipocytes, we found IL-8 and monocyte chemoattractant protein-1 
      (MCP-1) are constitutively secreted by both cell types and induced in response to 
      serum deprivation. MicroRNA profiling revealed the rapid induction of microRNA 
      132 (miR-132) in these cells when switched to serum-free medium. Furthermore, 
      miR-132 overexpression was sufficient to induce nuclear factor-kappaB 
      translocation, acetylation of p65, and production of IL-8 and MCP-1. Inhibitors 
      of miR-132 decreased acetylated p65 and partially inhibited the production of 
      IL-8 and MCP-1 induced by serum deprivation. MiR-132 was shown to inhibit silent 
      information regulator 1 (SirT1) expression through a miR-132 binding site in the 
      3'-untranslated region of SirT1. Thus, in response to nutritional availability, 
      induction of miR-132 decreases SirT1-mediated deacetylation of p65 leading to 
      activation of nuclear factor-kappaB and transcription of IL-8 and MCP-1 in 
      primary human preadipocytes and in vitro differentiated adipocytes.
FAU - Strum, Jay C
AU  - Strum JC
AD  - Department of Metabolic Discovery Technology Group, GlaxoSmithKline, Inc., 
      Research Triangle Park, North Carolina 27709, USA. jaystrum@msn.com
FAU - Johnson, Jennifer H
AU  - Johnson JH
FAU - Ward, James
AU  - Ward J
FAU - Xie, Hongbo
AU  - Xie H
FAU - Feild, John
AU  - Feild J
FAU - Hester, Austin
AU  - Hester A
FAU - Alford, Alexander
AU  - Alford A
FAU - Waters, K Michelle
AU  - Waters KM
LA  - eng
PT  - Journal Article
DEP - 20091009
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-8)
RN  - 0 (MIRN132 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 3.5.1.- (SIRT1 protein, human)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Adipocytes/metabolism
MH  - Adipose Tissue/cytology
MH  - Adult
MH  - Binding Sites
MH  - Chemokine CCL2/metabolism
MH  - Chemokines/*metabolism
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Interleukin-8/metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - *Nutritional Sciences
MH  - Sirtuin 1/metabolism/*physiology
MH  - Stem Cells/cytology
PMC - PMC5419165
EDAT- 2009/10/13 06:00
MHDA- 2010/02/18 06:00
PMCR- 2010/11/01
CRDT- 2009/10/13 06:00
PHST- 2009/10/13 06:00 [entrez]
PHST- 2009/10/13 06:00 [pubmed]
PHST- 2010/02/18 06:00 [medline]
PHST- 2010/11/01 00:00 [pmc-release]
AID - me.2009-0117 [pii]
AID - 10.1210/me.2009-0117 [doi]
PST - ppublish
SO  - Mol Endocrinol. 2009 Nov;23(11):1876-84. doi: 10.1210/me.2009-0117. Epub 2009 Oct 
      9.