PMID- 19822498
OWN - NLM
STAT- MEDLINE
DCOM- 20100301
LR  - 20111117
IS  - 1499-3872 (Print)
VI  - 8
IP  - 5
DP  - 2009 Oct
TI  - Effect of insulin on functional status of cord blood-derived dendritic cells and 
      on dendritic cell-induced CTL cytotoxicity against pancreatic cancer cell lines.
PG  - 529-34
AB  - BACKGROUND: Dendritic cells (DCs) are the most important antigen-presenting cells 
      in the human body, and DCs with different mature status possess different or even 
      opposite functions. This study was designed to explore the influence of insulin 
      on the functional status of cord blood-derived DCs and on DC-induced cytotoxic T 
      lymphocyte (CTL) activity against pancreatic cancer cell lines. METHODS: 
      Mononuclear cells were isolated from fresh cord blood. Interleukin-4 (IL-4) and 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) were used to induce or 
      stimulate the mononuclear cells. Insulin at different concentrations served to 
      modify DCs, and then DC morphology, number, and growth status were assessed. The 
      DC immunophenotype was detected with a flow cytometer. The IL-12 in DC 
      supernatant was determined by ELISA. DC functional status was evaluated by the 
      autologous mixed lymphocyte reaction. T lymphocytes were induced by 
      insulin-modified DCs to become CTLs. The CTL cytotoxicity against pancreatic 
      cancer cell lines was determined. RESULTS: Mononuclear cells from cord blood can 
      be differentiated into DCs by cytokine induction and insulin modification. With 
      the increase in insulin concentration (2.5-25 mg/L), the expression of DC HLA-DR, 
      CD1alpha, CD80, and CD83 was significantly increased, the DC ability to secrete 
      IL-12 was significantly improved, DC function to activate autologous lymphocytes 
      was significantly enhanced, and the cytotoxicity of CTLs induced by 
      insulin-modified DCs against pancreatic cancer cell lines was significantly 
      strengthened. CONCLUSIONS: Insulin may facilitate DC induction and maturation, 
      and improve the reproductive activity of autologous lymphocytes. The cytotoxicity 
      of CTLs induced by insulin-modified DCs against pancreatic cancer cell lines was 
      significantly enhanced. Insulin may serve as a factor modifying DCs and inducing 
      CTLs in vitro in insulin biotherapy.
FAU - Liu, Qiu-Liang
AU  - Liu QL
AD  - Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 
      450052, China.
FAU - Wang, Yi-Sheng
AU  - Wang YS
FAU - Wang, Jia-Xiang
AU  - Wang JX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Hepatobiliary Pancreat Dis Int
JT  - Hepatobiliary & pancreatic diseases international : HBPD INT
JID - 101151457
RN  - 0 (Insulin)
RN  - 0 (Interleukin-2)
SB  - IM
MH  - Adenocarcinoma/*pathology
MH  - Apoptosis/drug effects
MH  - Cell Communication/physiology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Dendritic Cells/cytology/*drug effects/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Fetal Blood/cytology
MH  - Humans
MH  - Immunophenotyping
MH  - Insulin/*pharmacology
MH  - Interleukin-2/metabolism
MH  - Pancreatic Neoplasms/*pathology
MH  - T-Lymphocytes, Cytotoxic/cytology/*drug effects/*physiology
EDAT- 2009/10/14 06:00
MHDA- 2010/03/02 06:00
CRDT- 2009/10/14 06:00
PHST- 2009/10/14 06:00 [entrez]
PHST- 2009/10/14 06:00 [pubmed]
PHST- 2010/03/02 06:00 [medline]
AID - 1282 [pii]
PST - ppublish
SO  - Hepatobiliary Pancreat Dis Int. 2009 Oct;8(5):529-34.