PMID- 19828768
OWN - NLM
STAT- MEDLINE
DCOM- 20100205
LR  - 20211020
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 16
IP  - 12
DP  - 2009 Dec
TI  - Detection of asymptomatic antigenemia in pigs infected by porcine reproductive 
      and respiratory syndrome virus (PRRSV) by a novel capture immunoassay with 
      monoclonal antibodies against the nucleocapsid protein of PRRSV.
PG  - 1822-8
LID - 10.1128/CVI.00244-09 [doi]
AB  - Routine surveillance for porcine reproductive and respiratory syndrome virus 
      (PRRSV) infections is crucial for the epidemiological control of this disease. 
      Antibody tests are widely used but cannot differentiate between vaccination and 
      reinfection. We developed a PRRSV antigen capture enzyme-linked immunosorbent 
      assay (ELISA) using well-characterized monoclonal antibodies (MAbs) raised 
      against the nucleocapsid (N) protein of North American and European PRRSV. This 
      antigen assay detected purified N protein from both genotypes at levels as low as 
      0.4 and 0.8 ng, respectively. The specificity and sensitivity of the N antigen 
      assay were evaluated with ground lung tissues from 8 PRRSV-infected and 16 
      healthy swine, and culture supernatants from six PRRSV isolates as well as other 
      swine viruses were confirmed by reverse transcriptase PCR (RT-PCR). Antigen 
      assays were positive in all eight infected tissues and with six different PRRSV 
      isolates, with no false positives among healthy tissues and other swine viruses 
      (i.e., pseudorabies and foot and mouth disease viruses). A number of sera, field 
      collected from 466 vaccinated and asymptomatic pigs in Guangdong, China, between 
      2008 and 2009, tested positive by the N antigen assay (12.45%), RT-PCR (15.02%), 
      and a commercial test for antibodies against PRRSV (78.97%). Of the 466 sera, 47 
      were positive by both antigen and RT-PCR tests, 11 by antigen test only, and 23 
      by RT-PCR only; the two assays had an overall agreement of 92.7%, indicating a 
      significant percentage of active PRRSV in asymptomatic pigs despite previous 
      immunization. These findings suggest that the antigen assay is a valuable field 
      tool for the epidemiological control of PRRSV that can be used for rapid 
      screening, particularly in asymptomatic animals.
FAU - Cai, Jian-Piao
AU  - Cai JP
AD  - Center for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, People's Republic of China.
FAU - Wang, Ya-Di
AU  - Wang YD
FAU - Tse, Herman
AU  - Tse H
FAU - Xiang, Hua
AU  - Xiang H
FAU - Yuen, Kwok-Yung
AU  - Yuen KY
FAU - Che, Xiao-Yan
AU  - Che XY
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091014
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Nucleocapsid Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antigens, Viral/*analysis/immunology
MH  - China/epidemiology
MH  - Environmental Monitoring/*methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epidemiological Monitoring
MH  - Lung/immunology/pathology/virology
MH  - Nucleocapsid Proteins/*analysis/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/*epidemiology/immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*isolation & 
      purification
MH  - Sensitivity and Specificity
MH  - Swine
PMC - PMC2786387
EDAT- 2009/10/16 06:00
MHDA- 2010/02/06 06:00
PMCR- 2010/06/01
CRDT- 2009/10/16 06:00
PHST- 2009/10/16 06:00 [entrez]
PHST- 2009/10/16 06:00 [pubmed]
PHST- 2010/02/06 06:00 [medline]
PHST- 2010/06/01 00:00 [pmc-release]
AID - CVI.00244-09 [pii]
AID - 0244-09 [pii]
AID - 10.1128/CVI.00244-09 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2009 Dec;16(12):1822-8. doi: 10.1128/CVI.00244-09. Epub 
      2009 Oct 14.