PMID- 19829698
OWN - NLM
STAT- MEDLINE
DCOM- 20100312
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 4
IP  - 10
DP  - 2009 Oct 15
TI  - Direct Repeat 6 from human herpesvirus-6B encodes a nuclear protein that forms a 
      complex with the viral DNA processivity factor p41.
PG  - e7457
LID - 10.1371/journal.pone.0007457 [doi]
LID - e7457
AB  - The SalI-L fragment from human herpesvirus 6A (HHV-6A) encodes a protein DR7 that 
      has been reported to produce fibrosarcomas when injected into nude mice, to 
      transform NIH3T3 cells, and to interact with and inhibit the function of p53. The 
      homologous gene in HHV-6B is dr6. Since p53 is deregulated in both HHV-6A and 
      -6B, we characterized the expression of dr6 mRNA and the localization of the 
      translated protein during HHV-6B infection of HCT116 cells. Expression of mRNA 
      from dr6 was inhibited by cycloheximide and partly by phosphonoacetic acid, a 
      known characteristic of herpesvirus early/late genes. DR6 could be detected as a 
      nuclear protein at 24 hpi and accumulated to high levels at 48 and 72 hpi. DR6 
      located in dots resembling viral replication compartments. Furthermore, a novel 
      interaction between DR6 and the viral DNA processivity factor, p41, could be 
      detected by confocal microscopy and by co-immunoprecipitation analysis. In 
      contrast, DR6 and p53 were found at distinct subcellular locations. Together, our 
      data imply a novel function of DR6 during HHV-6B replication.
FAU - Schleimann, Mariane H
AU  - Schleimann MH
AD  - Department of Medical Microbiology and Immunology, Aarhus University, Aarhus, 
      Denmark.
FAU - Moller, Janni M L
AU  - Moller JM
FAU - Kofod-Olsen, Emil
AU  - Kofod-Olsen E
FAU - Hollsberg, Per
AU  - Hollsberg P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091015
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Viral Proteins)
RN  - 138415-18-6 (p41 protein, Human herpesvirus 6)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Nucleus/metabolism/*virology
MH  - DNA, Viral/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - *Genes, Viral
MH  - Genome, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Mice
MH  - Microscopy, Confocal/methods
MH  - Protein Structure, Tertiary
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Tumor Suppressor Protein p53/metabolism
MH  - Viral Proteins/*genetics
MH  - *Virus Replication
PMC - PMC2759074
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2009/10/16 06:00
MHDA- 2010/03/13 06:00
PMCR- 2009/10/15
CRDT- 2009/10/16 06:00
PHST- 2009/08/14 00:00 [received]
PHST- 2009/09/18 00:00 [accepted]
PHST- 2009/10/16 06:00 [entrez]
PHST- 2009/10/16 06:00 [pubmed]
PHST- 2010/03/13 06:00 [medline]
PHST- 2009/10/15 00:00 [pmc-release]
AID - 09-PONE-RA-12261 [pii]
AID - 10.1371/journal.pone.0007457 [doi]
PST - epublish
SO  - PLoS One. 2009 Oct 15;4(10):e7457. doi: 10.1371/journal.pone.0007457.