PMID- 19834286
OWN - NLM
STAT- MEDLINE
DCOM- 20100128
LR  - 20190911
IS  - 1347-8648 (Electronic)
IS  - 1347-8613 (Linking)
VI  - 111
IP  - 2
DP  - 2009 Oct
TI  - Liquid perfluorochemical inhibits inducible nitric oxide synthase expression and 
      nitric oxide formation in lipopolysaccharide-treated RAW 264.7 macrophages.
PG  - 147-54
AB  - Partial liquid ventilation with various types of perfluorocarbon (PFC) has been 
      shown to be beneficial in treating acute lung injury, a clinical outcome that may 
      involve the anti-inflammatory activity of PFC. FC-77 is a type of PFC with 
      relatively higher vapor pressure and evaporative loss than other PFCs during 
      partial liquid ventilation. Overproduction of nitric oxide (NO) by inducible 
      nitric oxide synthase (iNOS) has been proposed to play a crucial role in the 
      pathogenesis of inflammatory diseases. However, whether the iNOS/NO pathway is 
      affected by FC-77 is unknown. Thus, the aim of this study was to investigate 
      whether FC-77 inhibits iNOS expression and NO production in lipopolysaccharide 
      (LPS)-treated RAW 264.7 macrophages. We found that treatment with FC-77 
      significantly attenuated LPS-induced iNOS expression/activity and production of 
      NO and reactive oxygen species (ROS). FC-77 also attenuated LPS-induced 
      pro-inflammatory cytokine formation, but enhanced interleukin-10 production. 
      Furthermore, the LPS-induced degradation of cytosolic IkappaB-alpha and 
      activation of nuclear transcription factor-kappaB (NF-kappaB) were also inhibited 
      by FC-77. In conclusion, the present study is the first to demonstrate that FC-77 
      decreases LPS-induced NO production in macrophages, which may be associated with 
      the suppression of pro-inflammatory cytokines, and ROS production, as well as 
      NF-kappaB activation. These results also provide a novel explanation for its 
      anti-inflammatory activity.
FAU - Chang, Li-Ping
AU  - Chang LP
AD  - Department of Radiation Oncology, Tri-Service General Hospital, Taipei, Taiwan.
FAU - Lai, Yuan-Shu
AU  - Lai YS
FAU - Wu, Chang-Jer
AU  - Wu CJ
FAU - Chou, Tz-Chong
AU  - Chou TC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Pharmacol Sci
JT  - Journal of pharmacological sciences
JID - 101167001
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Fluorocarbons)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nitrites)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (fluorocarbon 77)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 56NQM5UZT1 (2',7'-dichlorofluorescein)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Culture Media/analysis/chemistry
MH  - Cytokines/analysis/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Fluoresceins/metabolism
MH  - Fluorescent Dyes/metabolism
MH  - Fluorocarbons/*metabolism/pharmacology
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/cytology/*metabolism
MH  - Mice
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase Type II/*antagonists & inhibitors/genetics/metabolism
MH  - Nitrites/analysis
MH  - RNA, Messenger/analysis
MH  - Reactive Oxygen Species/metabolism
MH  - Time Factors
EDAT- 2009/10/17 06:00
MHDA- 2010/01/29 06:00
CRDT- 2009/10/17 06:00
PHST- 2009/10/17 06:00 [entrez]
PHST- 2009/10/17 06:00 [pubmed]
PHST- 2010/01/29 06:00 [medline]
AID - JST.JSTAGE/jphs/09043FP [pii]
AID - 10.1254/jphs.09043fp [doi]
PST - ppublish
SO  - J Pharmacol Sci. 2009 Oct;111(2):147-54. doi: 10.1254/jphs.09043fp.