PMID- 19835524
OWN - NLM
STAT- MEDLINE
DCOM- 20100205
LR  - 20131121
IS  - 1369-1635 (Electronic)
IS  - 0953-7104 (Linking)
VI  - 20
IP  - 8
DP  - 2009 Dec
TI  - Platelets of type 2 diabetic patients are characterized by high ATP content and 
      low mitochondrial membrane potential.
PG  - 588-93
LID - 10.3109/09537100903288422 [doi]
AB  - Platelet dysfunction plays a critical role in vascular complications of type 2 
      diabetes mellitus (T2DM). But the relationship between platelet hyperactivity and 
      its energy metabolic process remains unclear. This study was designed to explore 
      alterations of platelet mitochondrial ATP production and the possible mechanism. 
      A total of 39 T2DM patients without macrovascular and microvascular complications 
      and 32 normal controls were fasting sampled. Platelet ATP content was measured by 
      a high performance liquid chromatograph (HPLC). The flow cytometry technique was 
      adopted to evaluate mitochondrial membrane potential (DeltaPsim), the stored 
      force for platelet ATP production. Consequently, T2DM patients exhibited obvious 
      hyperglycemia, hyperlipidemia and hypertension, but normal platelet morphology. 
      Platelet ATP content was significantly higher in T2DM (0.032 +/- 0.010 
      micromol/10(9) platelets versus 0.017 +/- 0.006 micromol/10(9) platelets, p < 
      0.001) than in the control group. Interestingly, DeltaPsim was markedly decreased 
      in T2DM patients (0.79 +/- 0.18 versus 2.70 +/- 1.03, p < 0.001) compared with 
      normal controls. For whole subjects, a stepwise regression showed that plasma 
      glycated hemoglobin A1c (HbA1c) level positively correlated to platelet ATP 
      content (beta = 0.552, 95% CI = 0.072-1.451), and fasting plasma glucose (FPG) 
      level was negatively correlated to DeltaPsim (beta = -0.372, 95% CI = -0.471 to 
      -0.089). These data support that hyperglycemia of T2DM promotes platelet 
      mitochondria to generate more ATP, but decreases platelet mitochondrial 
      potential. The discordance between them requires further researches to elucidate.
FAU - Guo, Xinmin
AU  - Guo X
AD  - Department of Anatomy, Zhongshan School ofMedicine, Sun Yat-Sen University, 
      Guangzhou,Guangdong , Department of Endocrinology, Guangzhou RedCross Hospital, 
      Fourth Affiliated Hospital of JinanUniversity, Guangzhou, Guangdong, China
FAU - Wu, Jiajia
AU  - Wu J
FAU - Du, Junjun
AU  - Du J
FAU - Ran, Jianmin
AU  - Ran J
FAU - Xu, Jie
AU  - Xu J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
EIN - Platelets. 2010 Feb;21(1):84. Wu, Jiajia [added]; Du, Junjun [added]; Xu, Jie 
      [added];Li, Qingmei [removed]; Mei, Guangzhong [removed]; Lao, Gancheng[removed]
MH  - Adenosine Triphosphate/*metabolism
MH  - Aged
MH  - Animals
MH  - Blood Platelets/cytology/*metabolism
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Female
MH  - Humans
MH  - Hyperglycemia/metabolism
MH  - Male
MH  - Membrane Potential, Mitochondrial/*physiology
MH  - Middle Aged
EDAT- 2009/10/20 06:00
MHDA- 2010/02/06 06:00
CRDT- 2009/10/20 06:00
PHST- 2009/10/20 06:00 [entrez]
PHST- 2009/10/20 06:00 [pubmed]
PHST- 2010/02/06 06:00 [medline]
AID - 10.3109/09537100903288422 [pii]
AID - 10.3109/09537100903288422 [doi]
PST - ppublish
SO  - Platelets. 2009 Dec;20(8):588-93. doi: 10.3109/09537100903288422.