PMID- 19835760
OWN - NLM
STAT- MEDLINE
DCOM- 20100104
LR  - 20091019
IS  - 1095-9998 (Electronic)
IS  - 0740-0020 (Linking)
VI  - 26
IP  - 8
DP  - 2009 Dec
TI  - Analysis of the microflora in Tibetan kefir grains using denaturing gradient gel 
      electrophoresis.
PG  - 770-5
LID - 10.1016/j.fm.2009.04.009 [doi]
AB  - The microflora of Tibetan kefir grains was investigated by culture- independent 
      methods. Denaturing gradient gel electrophoresis (DGGE) of partially amplified 
      16S rRNA for bacteria and 26S rRNA for yeasts, followed by sequencing of the most 
      intense bands, showed that the dominant microorganisms were Pseudomonas sp., 
      Leuconostoc mesenteroides, Lactobacillus helveticus, Lactobacillus 
      kefiranofaciens, Lactococcus lactis, Lactobacillus kefiri, Lactobacillus casei, 
      Kazachstania unispora, Kluyveromyces marxianus, Saccharomyces cerevisiae, and 
      Kazachstania exigua. The bacterial communities between three kinds of Tibetan 
      kefir grains showed 78-84% similarity, and yeasts 80-92%. The microflora is held 
      together in the matrix of fibrillar material composed largely of a 
      water-insoluble polysaccharide.
FAU - Zhou, Jianzhong
AU  - Zhou J
AD  - Institute of Agro-product processing, Jiangsu Academy of Agricultural Sciences, 
      Nanjing 210014, China.
FAU - Liu, Xiaoli
AU  - Liu X
FAU - Jiang, Hanhu
AU  - Jiang H
FAU - Dong, Mingsheng
AU  - Dong M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090504
PL  - England
TA  - Food Microbiol
JT  - Food microbiology
JID - 8601127
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (RNA, ribosomal, 26S)
SB  - IM
MH  - Bacteria/classification/*isolation & purification
MH  - Cultured Milk Products/microbiology
MH  - Electrophoresis, Polyacrylamide Gel/*methods
MH  - *Food Microbiology
MH  - Gene Amplification
MH  - Microscopy, Electron, Scanning
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Bacterial/genetics
MH  - RNA, Fungal/genetics
MH  - RNA, Ribosomal/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sorghum/*microbiology/ultrastructure
MH  - Tibet
MH  - Yeasts/classification/*isolation & purification
EDAT- 2009/10/20 06:00
MHDA- 2010/01/05 06:00
CRDT- 2009/10/20 06:00
PHST- 2008/07/16 00:00 [received]
PHST- 2009/04/14 00:00 [revised]
PHST- 2009/04/19 00:00 [accepted]
PHST- 2009/10/20 06:00 [entrez]
PHST- 2009/10/20 06:00 [pubmed]
PHST- 2010/01/05 06:00 [medline]
AID - S0740-0020(09)00098-7 [pii]
AID - 10.1016/j.fm.2009.04.009 [doi]
PST - ppublish
SO  - Food Microbiol. 2009 Dec;26(8):770-5. doi: 10.1016/j.fm.2009.04.009. Epub 2009 
      May 4.