PMID- 19836022
OWN - NLM
STAT- MEDLINE
DCOM- 20100218
LR  - 20091102
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1216
IP  - 47
DP  - 2009 Nov 20
TI  - Trace determination of 10 beta-lactam antibiotics in environmental and food 
      samples by capillary liquid chromatography.
PG  - 8355-61
LID - 10.1016/j.chroma.2009.09.042 [doi]
AB  - A sensitive and reliable method using capillary HPLC with UV-diode array 
      detection (DAD) has been developed and validated for the trace determination of 
      residues of 10 beta-lactam antibiotics of human and veterinary use, in milk, 
      chicken meat and environmental water samples. The analytes included ampicillin, 
      amoxicillin, penicillin V, penicillin G, cloxacillin, oxacillin, dicloxacillin, 
      nafcillin, piperacillin and clavulanic acid. Legal levels are regulated by the EU 
      Council regulation 2377/90 in animal edible tissues for these compounds. For food 
      analysis, a solid-phase extraction (SPE) procedure consisting in a tandem of 
      Oasis HLB and Alumina N cartridges was applied for off-line preconcentration and 
      cleanup. For water analysis, the first step was only necessary. The limits of 
      detection for the studied compounds were between 0.04-0.06 microg l(-1) for water 
      samples and 0.80-1.40 microg l(-1) (or microg kg(-1)) in the case of foods 
      derived from animals. Average recoveries for fortified samples at different 
      concentration levels ranged between 82.9% and 98.2%, with relative standard 
      deviations (RSDs) lower than 9%. The method showed the advantages of capillary 
      HPLC for the detection of these widely applied antibiotics in different samples 
      at very low concentration levels.
FAU - Bailon-Perez, M I
AU  - Bailon-Perez MI
AD  - Department of Analytical Chemistry, Faculty of Sciences, Campus Fuentenueva, s/n, 
      E-18071 Granada, Spain.
FAU - Garcia-Campana, A M
AU  - Garcia-Campana AM
FAU - del Olmo-Iruela, M
AU  - del Olmo-Iruela M
FAU - Gamiz-Gracia, L
AU  - Gamiz-Gracia L
FAU - Cruces-Blanco, C
AU  - Cruces-Blanco C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090923
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Environmental Pollutants)
RN  - 0 (beta-Lactams)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/chemistry
MH  - Chickens
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Environmental Pollutants/*analysis
MH  - Food Analysis/*methods
MH  - Fresh Water/*chemistry
MH  - Linear Models
MH  - Meat/analysis
MH  - Milk/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - beta-Lactams/*analysis/chemistry
EDAT- 2009/10/20 06:00
MHDA- 2010/02/19 06:00
CRDT- 2009/10/20 06:00
PHST- 2009/07/09 00:00 [received]
PHST- 2009/09/14 00:00 [revised]
PHST- 2009/09/17 00:00 [accepted]
PHST- 2009/10/20 06:00 [entrez]
PHST- 2009/10/20 06:00 [pubmed]
PHST- 2010/02/19 06:00 [medline]
AID - S0021-9673(09)01414-9 [pii]
AID - 10.1016/j.chroma.2009.09.042 [doi]
PST - ppublish
SO  - J Chromatogr A. 2009 Nov 20;1216(47):8355-61. doi: 10.1016/j.chroma.2009.09.042. 
      Epub 2009 Sep 23.