PMID- 19837871
OWN - NLM
STAT- MEDLINE
DCOM- 20100119
LR  - 20211203
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 150
IP  - 12
DP  - 2009 Dec
TI  - Response to carbohydrate and fat refeeding in the expression of genes involved in 
      nutrient partitioning and metabolism: striking effects on fibroblast growth 
      factor-21 induction.
PG  - 5341-50
LID - 10.1210/en.2009-0466 [doi]
AB  - This study aimed to assess the effects of carbohydrate (CHO) and fat intake on 
      the expression of key genes related with nutrient partitioning and metabolism in 
      main tissues involved in energy metabolism (white adipose tissue, liver, and 
      skeletal muscle). Rats were studied under different conditions: feeding state, 24 
      h fasting, and 12 h refeeding after 24 h fasting with isocaloric amounts of CHO 
      or fat. Fat, but not CHO, refeeding was associated with an increase in serum and 
      liver triglyceride content. Main changes in gene expression elicited by CHO 
      compared with fat refeeding were: 1) higher expression levels of genes related 
      with lipogenesis (PPARgamma2, ChREBP, FAS), glucose uptake and metabolism (GLUT4, 
      HKII), fatty acid uptake (LPL, CD36), and lipolysis (ATGL, HSL) in white adipose 
      tissue; 2) higher expression levels of genes related with lipogenesis (FAS, SCD1) 
      but lower ones related with fatty acid uptake (CD36) and oxidation (PPARalpha, 
      CPT1, PDK4) in liver; and 3) higher expression levels of GLUT4 but lower ones 
      related with fatty acid oxidation (PDK4 and UCP3) in muscle. It is worth 
      mentioning that both CHO and fat refeeding resulted in a robust increase in both 
      hepatic mRNA and circulating levels of fibroblast growth factor-21, compared with 
      fasted levels. In summary, these results, showing marked differences in gene 
      expression after CHO and fat refeeding, can explain diet-associated differences 
      in fuel handling and partitioning between tissues; in addition, a role of 
      fibroblast growth factor-21 in metabolic adaptations, not only in the ketotic 
      state but also to face an unbalanced nutritional situation, is suggested.
FAU - Sanchez, J
AU  - Sanchez J
AD  - University of the Balearic Islands and Centro de Investigacion Biomedica en Red 
      Fisiopatologia de la Obesidad y Nutricion, Palma de Mallorca 07122, Spain.
FAU - Palou, A
AU  - Palou A
FAU - Pico, C
AU  - Pico C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091016
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Blood Glucose)
RN  - 0 (CD36 Antigens)
RN  - 0 (Cd36 protein, rat)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (Pdk4 protein, rat)
RN  - 0 (Pyruvate Dehydrogenase Acetyl-Transferring Kinase)
RN  - 0 (Slc2a4 protein, rat)
RN  - 0 (Triglycerides)
RN  - 0 (fibroblast growth factor 21)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Adipose Tissue, White/drug effects/metabolism
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - CD36 Antigens/genetics
MH  - Dietary Carbohydrates/administration & dosage/*pharmacology
MH  - Dietary Fats/administration & dosage/*pharmacology
MH  - Energy Metabolism/*genetics
MH  - Fibroblast Growth Factors/blood/genetics/metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Glucose Transporter Type 4/genetics
MH  - Insulin/blood
MH  - Intra-Abdominal Fat/drug effects/metabolism
MH  - Lipogenesis/genetics
MH  - Lipolysis/genetics
MH  - Liver/drug effects/metabolism
MH  - Male
MH  - Muscle, Skeletal/drug effects/metabolism
MH  - Nutritional Physiological Phenomena/drug effects
MH  - Protein Serine-Threonine Kinases/genetics
MH  - Pyruvate Dehydrogenase Acetyl-Transferring Kinase
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Triglycerides/blood
EDAT- 2009/10/20 06:00
MHDA- 2010/01/20 06:00
CRDT- 2009/10/20 06:00
PHST- 2009/10/20 06:00 [entrez]
PHST- 2009/10/20 06:00 [pubmed]
PHST- 2010/01/20 06:00 [medline]
AID - en.2009-0466 [pii]
AID - 10.1210/en.2009-0466 [doi]
PST - ppublish
SO  - Endocrinology. 2009 Dec;150(12):5341-50. doi: 10.1210/en.2009-0466. Epub 2009 Oct 
      16.