PMID- 19838331
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100520
LR  - 20211020
IS  - 1177-6250 (Electronic)
IS  - 1177-6250 (Linking)
VI  - 3
DP  - 2009 Feb 19
TI  - RNAi-mediated knockdown showing impaired cell survival in Drosophila wing 
      imaginal disc.
PG  - 11-20
AB  - The genetically amenable organism Drosophila melanogaster has been estimated to 
      have 14,076 protein coding genes in the genome, according to the flybase release 
      note R5.13 (http://flybase.bio.indiana.edu/static_pages/docs/release_notes.html). 
      Recent application of RNA interference (RNAi) to the study of developmental 
      biology in Drosophila has enabled us to carry out a systematic investigation of 
      genes affecting various specific phenotypes. In order to search for genes 
      supporting cell survival, we conducted an immunohistochemical examination in 
      which the RNAi of 2,497 genes was independently induced within the dorsal 
      compartment of the wing imaginal disc. Under these conditions, the activities of 
      a stress-activated protein kinase JNK (c-Jun N-terminal kinase) and 
      apoptosis-executing factor Caspase-3 were monitored. Approximately half of the 
      genes displayed a strong JNK or Caspase-3 activation when their RNAi was induced. 
      Most of the JNK activation accompanied Caspase-3 activation, while the opposite 
      did not hold true. Interestingly, the area activating Caspase-3 was more broadly 
      seen than that activating JNK, suggesting that JNK is crucial for induction of 
      non-autonomous apoptosis in many cases. Furthermore, the RNAi of essential 
      factors commonly regulating transcription and translation showed a severe and 
      cell-autonomous apoptosis but also elicited another apoptosis at an adjacent area 
      in a non-autonomous way. We also found that the frequency of apoptosis varies 
      depending on the tissues.
FAU - Umemori, Makoto
AU  - Umemori M
AD  - Department of Biology, Graduate School of Science, Kobe University, Kobe, Japan.
FAU - Habara, Okiko
AU  - Habara O
FAU - Iwata, Tatsunori
AU  - Iwata T
FAU - Maeda, Kousuke
AU  - Maeda K
FAU - Nishinoue, Kana
AU  - Nishinoue K
FAU - Okabe, Atsushi
AU  - Okabe A
FAU - Takemura, Masahiko
AU  - Takemura M
FAU - Takahashi, Kuniaki
AU  - Takahashi K
FAU - Saigo, Kaoru
AU  - Saigo K
FAU - Ueda, Ryu
AU  - Ueda R
FAU - Adachi-Yamada, Takashi
AU  - Adachi-Yamada T
LA  - eng
PT  - Journal Article
DEP - 20090219
PL  - United States
TA  - Gene Regul Syst Bio
JT  - Gene regulation and systems biology
JID - 101312647
PMC - PMC2758276
OTO - NOTNLM
OT  - Drosophila
OT  - JNK caspase-3
OT  - RNAi apoptosis
EDAT- 2009/10/20 06:00
MHDA- 2009/10/20 06:01
PMCR- 2009/02/19
CRDT- 2009/10/20 06:00
PHST- 2009/10/20 06:00 [entrez]
PHST- 2009/10/20 06:00 [pubmed]
PHST- 2009/10/20 06:01 [medline]
PHST- 2009/02/19 00:00 [pmc-release]
AID - grsb-2009-011 [pii]
AID - 10.4137/grsb.s2100 [doi]
PST - epublish
SO  - Gene Regul Syst Bio. 2009 Feb 19;3:11-20. doi: 10.4137/grsb.s2100.